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10.1371/journal.pone.0139335 http://scihub22266oqcxt.onion/10.1371/journal.pone.0139335 C4591338!4591338 !26426529     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26426529 &cmd=llinks): Failed to open stream: HTTP request failed! 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Peripheral Nerve Transplantation Combined with Acidic Fibroblast Growth Factor and Chondroitinase Induces Regeneration and Improves Urinary Function in Complete Spinal Cord Transected Adult Mice |pdf=|usr=}}{{26426529 }} gab.com Text Peripheral Nerve Transplantation Combined with Acidic Fibroblast Growth Factor and Chondroitinase Induces Regeneration and Improves Urinary Function in Complete Spinal Cord Transected Adult Mice JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26426529 #FOAvip The loss of lower urinary tract (LUT) control is a ubiquitous consequence of a complete spinal cord injury, attributed to a lack of regeneration of supraspinal pathways controlling the bladder. Previous work in our lab has utilized a combinatorial therapy of peripheral nerve autografts (PNG), acidic fibroblast growth factor (aFGF), and chondroitinase ABC (ChABC) to treat a complete T8 spinal cord transection in the adult rat, resulting in supraspinal control of bladder function. In the present study we extended these findings by examining the use of the combinatorial PNG+aFGF+ChABC treatment in a T8 transected mouse model, which more closely models human urinary deficits following spinal cord injury. Cystometry analysis and external urethral sphincter electromyograms reveal that treatment with PNG+aFGF+ChABC reduced bladder weight, improved bladder and external urethral sphincter histology, and significantly enhanced LUT function, resulting in more efficient voiding. Treated mice's injured spinal cord also showed a reduction in collagen scaring, and regeneration of serotonergic and tyrosine hydroxylase-positive axons across the lesion and into the distal spinal cord. Regeneration of serotonin axons correlated with LUT recovery. These results suggest that our mouse model of LUT dysfunction recapitulates the results found in the rat model and may be used to further investigate genetic contributions to regeneration failure. Twit Text FOAVip Peripheral Nerve Transplantation Combined with Acidic Fibroblast Growth Factor and Chondroitinase Induces Regeneration and Improves Urinary Function in Complete Spinal Cord Transected Adult Mice ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26426529 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26426529 #FOAvip English Wikipedia <ref>{{Cite pmid|26426529 }}</ref> Peripheral Nerve Transplantation Combined with Acidic Fibroblast Growth Factor and Chondroitinase Induces Regeneration and Improves Urinary Function in Complete Spinal Cord Transected Adult Mice #MMPMID26426529 DePaul MA ; Lin CY ; Silver J ; Lee YS PLoS One 2015[]; 10 (10 ): e0139335 PMID26426529 show ga The loss of lower urinary tract (LUT) control is a ubiquitous consequence of a complete spinal cord injury, attributed to a lack of regeneration of supraspinal pathways controlling the bladder. Previous work in our lab has utilized a combinatorial therapy of peripheral nerve autografts (PNG), acidic fibroblast growth factor (aFGF), and chondroitinase ABC (ChABC) to treat a complete T8 spinal cord transection in the adult rat, resulting in supraspinal control of bladder function. In the present study we extended these findings by examining the use of the combinatorial PNG+aFGF+ChABC treatment in a T8 transected mouse model, which more closely models human urinary deficits following spinal cord injury. Cystometry analysis and external urethral sphincter electromyograms reveal that treatment with PNG+aFGF+ChABC reduced bladder weight, improved bladder and external urethral sphincter histology, and significantly enhanced LUT function, resulting in more efficient voiding. Treated mice's injured spinal cord also showed a reduction in collagen scaring, and regeneration of serotonergic and tyrosine hydroxylase-positive axons across the lesion and into the distal spinal cord. Regeneration of serotonin axons correlated with LUT recovery. These results suggest that our mouse model of LUT dysfunction recapitulates the results found in the rat model and may be used to further investigate genetic contributions to regeneration failure. |Animals [MESH] |Chondroitin ABC Lyase/*administration & dosage [MESH] |Disease Models, Animal [MESH] |Electromyography [MESH] |Female [MESH] |Fibroblast Growth Factor 1/*administration & dosage [MESH] |Humans [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Nerve Regeneration/*physiology [MESH] |Peripheral Nerves/transplantation [MESH] |Rats [MESH] |Recovery of Function [MESH] |Spinal Cord Injuries/*physiopathology/*rehabilitation [MESH] |Tyrosine 3-Monooxygenase/metabolism [MESH] |Urinary Bladder/innervation/*physiology [MESH] |Urination/*drug effects [MESH]Peripheral Nerve Transplantation Combined with Acidic Fibroblast Growt(epmc).pdf DeepDyve Pubget Overpricing