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10.1007/s11010-015-2509-9 http://scihub22266oqcxt.onion/10.1007/s11010-015-2509-9 C4589559!4589559 !26169986     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 284.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 284.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 284.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26169986 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Cell+Biochem 2015 ; 409 (1-2 ): 33-43 Nephropedia Template TP {{tp|p=26169986 |t=2015. Cucurbitacin D induces cell cycle arrest and apoptosis by inhibiting STAT3 and NF-?B signaling in doxorubicin-resistant human breast carcinoma (MCF7/ADR) cells |pdf=|usr=}}{{26169986 }} gab.com Text Cucurbitacin D induces cell cycle arrest and apoptosis by inhibiting STAT3 and NF- B signaling in doxorubicin-resistant human breast carcinoma (MCF7/ADR) cells JO: Mol Cell Biochem http://www.kidney.de/pget.php?&tw=1&pmid=26169986 #FOAvip Breast cancer is the most common cancer for women and is a major cause of mortality in women. Doxorubicin is a generally used chemotherapy drug for breast cancer. However, multidrug resistance of breast cancer interferes with the chemotherapy. We examined whether cucurbitacin D affects doxorubicin resistance of MCF7/ADR breast cancer cells. Cell viability was measured by MTT assay. Levels of p-STAT3, p-NF-?B, I?B, and caspases were measured by Western blot analysis. Nuclear staining of Stat3 and NF-?B was measured by immunocytochemistry. STAT3 and NF-?B transcriptional activity was detected by STAT3 and NF-?B luciferase reporter gene assays. Analysis of cell cycle arrest was performed by flow cytometry. Induction of apoptosis by cucurbitacin D was measured by Annexin V-FITC/propidium iodide assay. More than 90% of MCF7/ADR cells lived upon treatment with doxorubicin for 24 h. However, upon treatment with cucurbitacin D, cell death was more than 60%. Co-administration of cucurbitacin D and doxorubicin induced apoptosis, and G2/M cell cycle arrest, and inhibited upregulated Stat3 by doxorubicin on MCF7/ADR cells. Additionally, cucurbitacin D led to an increase in the I?B? level in the cytosol and a decrease in the p-NF-?B level in the nucleus. Finally, cucurbitacin D inhibited translocation of Stat3 and NF-?B and decreased transcriptional activity in the nucleus. Cucurbitacin D decreases cell proliferation and induces apoptosis by inhibiting Stat3 and NF-?B signaling in doxorubicin-resistant breast cancer cells. Cucurbitacin D could be used as a useful compound to treat adriamycin-resistant patients. Twit Text FOAVip Cucurbitacin D induces cell cycle arrest and apoptosis by inhibiting STAT3 and NF- B signaling in doxorubicin-resistant human breast carcinoma (MCF7/ADR) cells ????Mol Cell Biochem http://www.kidney.de/pget.php?&tw=1&pmid=26169986 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26169986 #FOAvip English Wikipedia <ref>{{Cite pmid|26169986 }}</ref> Cucurbitacin D induces cell cycle arrest and apoptosis by inhibiting STAT3 and NF-?B signaling in doxorubicin-resistant human breast carcinoma (MCF7/ADR) cells #MMPMID26169986 Ku JM ; Kim SR ; Hong SH ; Choi HS ; Seo HS ; Shin YC ; Ko SG Mol Cell Biochem 2015[Nov]; 409 (1-2 ): 33-43 PMID26169986 show ga Breast cancer is the most common cancer for women and is a major cause of mortality in women. Doxorubicin is a generally used chemotherapy drug for breast cancer. However, multidrug resistance of breast cancer interferes with the chemotherapy. We examined whether cucurbitacin D affects doxorubicin resistance of MCF7/ADR breast cancer cells. Cell viability was measured by MTT assay. Levels of p-STAT3, p-NF-?B, I?B, and caspases were measured by Western blot analysis. Nuclear staining of Stat3 and NF-?B was measured by immunocytochemistry. STAT3 and NF-?B transcriptional activity was detected by STAT3 and NF-?B luciferase reporter gene assays. Analysis of cell cycle arrest was performed by flow cytometry. Induction of apoptosis by cucurbitacin D was measured by Annexin V-FITC/propidium iodide assay. More than 90% of MCF7/ADR cells lived upon treatment with doxorubicin for 24 h. However, upon treatment with cucurbitacin D, cell death was more than 60%. Co-administration of cucurbitacin D and doxorubicin induced apoptosis, and G2/M cell cycle arrest, and inhibited upregulated Stat3 by doxorubicin on MCF7/ADR cells. Additionally, cucurbitacin D led to an increase in the I?B? level in the cytosol and a decrease in the p-NF-?B level in the nucleus. Finally, cucurbitacin D inhibited translocation of Stat3 and NF-?B and decreased transcriptional activity in the nucleus. Cucurbitacin D decreases cell proliferation and induces apoptosis by inhibiting Stat3 and NF-?B signaling in doxorubicin-resistant breast cancer cells. Cucurbitacin D could be used as a useful compound to treat adriamycin-resistant patients. |Apoptosis/*drug effects [MESH] |Breast Neoplasms/*drug therapy/pathology [MESH] |Caspases/metabolism [MESH] |Cell Line, Tumor [MESH] |Cell Proliferation/drug effects [MESH] |Cell Survival/drug effects [MESH] |Doxorubicin/*pharmacology [MESH] |Drug Resistance, Multiple [MESH] |Drug Resistance, Neoplasm [MESH] |Female [MESH] |G2 Phase Cell Cycle Checkpoints/*drug effects [MESH] |Gene Expression Regulation, Neoplastic/drug effects [MESH] |Humans [MESH] |I-kappa B Proteins/metabolism [MESH] |MCF-7 Cells [MESH] |NF-KappaB Inhibitor alpha [MESH] |NF-kappa B/*antagonists & inhibitors/metabolism [MESH] |STAT3 Transcription Factor/*antagonists & inhibitors/metabolism [MESH] |Signal Transduction/drug effects [MESH]Cucurbitacin D induces cell cycle arrest and apoptosis by inhibiting S(epmc).pdf DeepDyve Pubget Overpricing