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Relating plaque morphology to respiratory syncytial virus subgroup, viral load,
and disease severity in children
#MMPMID26107392
Kim YI
; Murphy R
; Majumdar S
; Harrison LG
; Aitken J
; DeVincenzo JP
Pediatr Res
2015[Oct]; 78
(4
): 380-8
PMID26107392
show ga
BACKGROUND: Viral culture plaque morphology in human cell lines are markers for
growth capability and cytopathic effect, and have been used to assess viral
fitness and select preattenuation candidates for live viral vaccines. We
classified respiratory syncytial virus (RSV) plaque morphology and analyzed the
relationship between plaque morphology as compared to subgroup, viral load and
clinical severity of infection in infants and children. METHODS: We obtained
respiratory secretions from 149 RSV-infected children. Plaque morphology and
viral load was assessed within the first culture passage in HEp-2 cells. Viral
load was measured by polymerase chain reaction (PCR), as was RSV subgroup.
Disease severity was determined by hospitalization, length of stay, intensive
care requirement, and respiratory failure. RESULTS: Plaque morphology varied
between individual subjects; however, similar results were observed among viruses
collected from upper and lower respiratory tracts of the same subject.
Significant differences in plaque morphology were observed between RSV subgroups.
No correlations were found among plaque morphology and viral load. Plaque
morphology did not correlate with disease severity. CONCLUSION: Plaque morphology
measures parameters that are viral-specific and independent of the human host.
Morphologies vary between patients and are related to RSV subgroup. In HEp-2
cells, RSV plaque morphology appears unrelated to disease severity in
RSV-infected children.
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