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2015 ; 7
(3
): 122-36
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Anti-Apoptotic Gene Delivery with cyclo-(d-Trp-Tyr) Peptide Nanotube via Eye Drop
Following Corneal Epithelial Debridement
#MMPMID26193308
Lee YH
; Chang SF
; Liaw J
Pharmaceutics
2015[Jul]; 7
(3
): 122-36
PMID26193308
show ga
Corneal keratocyte apoptosis triggered by cornel debridement is one mechanism of
corneal disorders. In this study, the feasibility of cyclo-(d-Trp-Tyr) peptide
nanotubes (PNTs) as carriers of caspase 3 silence shRNA delivery was assessed. A
model of epithelial injury by epithelial debridement was applied to investigate
the feasibility of PNTs as gene delivery carriers on corneal injury. First, the
PNTs were found within 2 ?m in length and 300 nm in width by an atomic force
microscope and confocal laser microscope system. Plasmid DNAs were observed to be
associated with PNTs by atomic force microscope and confocal laser scanning
microscope. The plasmids were associated with tyrosine of PNTs with a binding
constant of 2.7 × 108 M-1. The stability of plasmid DNA with PNTs against the
DNase was found at 60 min. Using thioflavin T pre-stained PNTs on the corneal eye
drop delivery, the distribution of PNTs was in the epithelial and stroma regions.
After corneal debridement, the rhodamine-labeled plasmid DNA and thioflavin T
pre-stained PNTs were also delivered and could be observed in the stroma of
cornea. PNTs complexed with anti-apoptotic plasmid caspase 3 silencing shRNA eye
drop delivery decreased 41% of caspase 3 activity after the first dose by caspase
3 activity and Western blot analysis.