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10.1167/iovs.13-12410 http://scihub22266oqcxt.onion/10.1167/iovs.13-12410 C4586970!4586970 !24398102     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24398102 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Invest+Ophthalmol+Vis+Sci 2014 ; 55 (3 ): 1637-46 Nephropedia Template TP {{tp|p=24398102 |t=2014. Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1? in human corneal limbal epithelial cells |pdf=|usr=}}{{24398102 }} gab.com Text Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1 in human corneal limbal epithelial cells JO: Invest Ophthalmol Vis Sci http://www.kidney.de/pget.php?&tw=1&pmid=24398102 #FOAvip PURPOSE: Corneal neovascularization (NV) is a sight-threatening condition often associated with infection, inflammation, prolonged contact lens use, corneal burns, and acute corneal graft rejection. Macrophages recruited to the cornea release nitric oxide (NO) and superoxide anion (O2(-)), which react together to form the highly toxic molecule peroxynitrite (ONOO(-)). The role of ONOO(-) in upregulating multiple angiogenic factors in cultured human corneal limbal epithelial (HCLE) cells was investigated. METHODS: Human corneal limbal epithelial cells were incubated with 500 ?M of ONOO(-) donor for various times. VEGF-A, BFGF, and hypoxic-inducible factor-alpha (HIF-1?) were investigated via Western blot and RT-PCR was performed for VEGF. Functional assays using human umbilical vein endothelial cells (HUVEC) used conditioned media from ONOO(-)-exposed HCLE cells. Secreted VEGF from conditioned media was detected and analyzed using ELISA. RESULTS: Increased angiogenic factors were observed as early as 4 hours after HCLE exposure to ONOO(-). HIF-1 expression was seen at 4, 6, and 8 hours post-ONOO(-) exposure (P < 0.05). BFGF expression was elevated at 4 hours and peaked at 8 hours after treatment with ONOO(-) (P < 0.005). Increased VEGF-A gene expression was observed at 6 and 8 hours post-ONOO(-) treatment. Functional assays using conditioned media showed increased HUVEC migration and tube formation. CONCLUSIONS: Exposure to elevated extracellular concentrations of ONOO(-) results in upregulation of angiogenic factors in HCLE cells. It is possible that, in the setting of inflammation or infection, that exposure to ONOO(-) could be one contributor to the complex initiators of corneal NV. Validation in vivo would identify an additional potential control point for corneal NV. Twit Text FOAVip Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1 in human corneal limbal epithelial cells ????Invest Ophthalmol Vis Sci http://www.kidney.de/pget.php?&tw=1&pmid=24398102 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24398102 #FOAvip English Wikipedia <ref>{{Cite pmid|24398102 }}</ref> Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1? in human corneal limbal epithelial cells #MMPMID24398102 Ashki N ; Chan AM ; Qin Y ; Wang W ; Kiyohara M ; Lin L ; Braun J ; Wadehra M ; Gordon LK Invest Ophthalmol Vis Sci 2014[Mar]; 55 (3 ): 1637-46 PMID24398102 show ga PURPOSE: Corneal neovascularization (NV) is a sight-threatening condition often associated with infection, inflammation, prolonged contact lens use, corneal burns, and acute corneal graft rejection. Macrophages recruited to the cornea release nitric oxide (NO) and superoxide anion (O2(-)), which react together to form the highly toxic molecule peroxynitrite (ONOO(-)). The role of ONOO(-) in upregulating multiple angiogenic factors in cultured human corneal limbal epithelial (HCLE) cells was investigated. METHODS: Human corneal limbal epithelial cells were incubated with 500 ?M of ONOO(-) donor for various times. VEGF-A, BFGF, and hypoxic-inducible factor-alpha (HIF-1?) were investigated via Western blot and RT-PCR was performed for VEGF. Functional assays using human umbilical vein endothelial cells (HUVEC) used conditioned media from ONOO(-)-exposed HCLE cells. Secreted VEGF from conditioned media was detected and analyzed using ELISA. RESULTS: Increased angiogenic factors were observed as early as 4 hours after HCLE exposure to ONOO(-). HIF-1 expression was seen at 4, 6, and 8 hours post-ONOO(-) exposure (P < 0.05). BFGF expression was elevated at 4 hours and peaked at 8 hours after treatment with ONOO(-) (P < 0.005). Increased VEGF-A gene expression was observed at 6 and 8 hours post-ONOO(-) treatment. Functional assays using conditioned media showed increased HUVEC migration and tube formation. CONCLUSIONS: Exposure to elevated extracellular concentrations of ONOO(-) results in upregulation of angiogenic factors in HCLE cells. It is possible that, in the setting of inflammation or infection, that exposure to ONOO(-) could be one contributor to the complex initiators of corneal NV. Validation in vivo would identify an additional potential control point for corneal NV. |*Up-Regulation [MESH] |Blotting, Western [MESH] |Cells, Cultured [MESH] |Corneal Neovascularization/*genetics/metabolism/pathology [MESH] |Enzyme-Linked Immunosorbent Assay [MESH] |Fibroblast Growth Factor 2/biosynthesis/*genetics [MESH] |Flow Cytometry [MESH] |Humans [MESH] |Hypoxia-Inducible Factor 1, alpha Subunit/biosynthesis/*genetics [MESH] |Limbus Corneae/drug effects/*metabolism/pathology [MESH] |Peroxynitrous Acid/*pharmacology [MESH] |RNA/genetics [MESH] |Real-Time Polymerase Chain Reaction [MESH] |Reverse Transcriptase Polymerase Chain Reaction [MESH]Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1? (epmc).pdf DeepDyve Pubget Overpricing