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10.1073/pnas.1511270112 http://scihub22266oqcxt.onion/10.1073/pnas.1511270112 C4586852!4586852 !26324941     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26324941 &cmd=llinks): Failed to open stream: HTTP request failed! 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Complexity of the human memory B-cell compartment is determined by the versatility of clonal diversification in germinal centers |pdf=|usr=}}{{26324941 }} gab.com Text Complexity of the human memory B-cell compartment is determined by the versatility of clonal diversification in germinal centers JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26324941 #FOAvip Our knowledge about the clonal composition and intraclonal diversity of the human memory B-cell compartment and the relationship between memory B-cell subsets is still limited, although these are central issues for our understanding of adaptive immunity. We performed a deep sequencing analysis of rearranged immunoglobulin (Ig) heavy chain genes from biological replicates, covering more than 100,000 memory B lymphocytes from two healthy adults. We reveal a highly similar B-cell receptor repertoire among the four main human IgM(+) and IgG(+) memory B-cell subsets. Strikingly, in both donors, 45% of sequences could be assigned to expanded clones, demonstrating that the human memory B-cell compartment is characterized by many, often very large, B-cell clones. Twenty percent of the clones consisted of class switched and IgM(+)(IgD(+)) members, a feature that correlated significantly with clone size. Hence, we provide strong evidence that the vast majority of Ig mutated B cells--including IgM(+)IgD(+)CD27(+) B cells--are post-germinal center (GC) memory B cells. Clone members showed high intraclonal sequence diversity and high intraclonal versatility in Ig class and IgG subclass composition, with particular patterns of memory B-cell clone generation in GC reactions. In conclusion, GC produce amazingly large, complex, and diverse memory B-cell clones, equipping the human immune system with a versatile and highly diverse compartment of IgM(+)(IgD(+)) and class-switched memory B cells. Twit Text FOAVip Complexity of the human memory B-cell compartment is determined by the versatility of clonal diversification in germinal centers ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26324941 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26324941 #FOAvip English Wikipedia <ref>{{Cite pmid|26324941 }}</ref> Complexity of the human memory B-cell compartment is determined by the versatility of clonal diversification in germinal centers #MMPMID26324941 Budeus B ; Schweigle de Reynoso S ; Przekopowitz M ; Hoffmann D ; Seifert M ; Küppers R Proc Natl Acad Sci U S A 2015[Sep]; 112 (38 ): E5281-9 PMID26324941 show ga Our knowledge about the clonal composition and intraclonal diversity of the human memory B-cell compartment and the relationship between memory B-cell subsets is still limited, although these are central issues for our understanding of adaptive immunity. We performed a deep sequencing analysis of rearranged immunoglobulin (Ig) heavy chain genes from biological replicates, covering more than 100,000 memory B lymphocytes from two healthy adults. We reveal a highly similar B-cell receptor repertoire among the four main human IgM(+) and IgG(+) memory B-cell subsets. Strikingly, in both donors, 45% of sequences could be assigned to expanded clones, demonstrating that the human memory B-cell compartment is characterized by many, often very large, B-cell clones. Twenty percent of the clones consisted of class switched and IgM(+)(IgD(+)) members, a feature that correlated significantly with clone size. Hence, we provide strong evidence that the vast majority of Ig mutated B cells--including IgM(+)IgD(+)CD27(+) B cells--are post-germinal center (GC) memory B cells. Clone members showed high intraclonal sequence diversity and high intraclonal versatility in Ig class and IgG subclass composition, with particular patterns of memory B-cell clone generation in GC reactions. In conclusion, GC produce amazingly large, complex, and diverse memory B-cell clones, equipping the human immune system with a versatile and highly diverse compartment of IgM(+)(IgD(+)) and class-switched memory B cells. |*Immunologic Memory [MESH] |Adaptive Immunity [MESH] |Adult [MESH] |B-Lymphocytes/*cytology [MESH] |Cell Separation [MESH] |Computational Biology [MESH] |DNA Mutational Analysis [MESH] |Germinal Center/*cytology [MESH] |Humans [MESH] |Immunoglobulin Class Switching [MESH] |Immunoglobulin D/immunology [MESH] |Immunoglobulin Heavy Chains/genetics [MESH] |Immunoglobulin M/immunology [MESH] |Immunoglobulin Variable Region/genetics [MESH] |Male [MESH] |Mutation [MESH]Complexity of the human memory B-cell compartment is determined by the(epmc).pdf DeepDyve Pubget Overpricing