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2015 ; 5
(ä): 14469
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Osmotic/ionic status of body fluids in the euryhaline cephalopod suggest possible
parallel evolution of osmoregulation
#MMPMID26403952
Sakamoto T
; Ogawa S
; Nishiyama Y
; Akada C
; Takahashi H
; Watanabe T
; Minakata H
; Sakamoto H
Sci Rep
2015[Sep]; 5
(ä): 14469
PMID26403952
show ga
Acclimation from marine to dilute environments constitutes among the dramatic
evolutionary transitions in the history of life. Such adaptations have evolved in
multiple lineages, but studies of the blood/hemolymph homeostasis mechanisms are
limited to those using evolutionarily advanced Deuterostome (chordates) and
Ecdysozoa (crustaceans). Here, we examined hemolymph homeostasis in the advanced
Lophotrochozoa/mollusc, the other unexplored taxa, and its possible regulation by
the vasopressin/oxytocin superfamily peptides known to be implicated in fluid
homeostasis in Chordata and Arthropoda. The hemolymph osmotic and ionic status in
the euryhaline cephalopod (Octopus ocellatus) following transfer from 30-ppt
normal seawater to 20 ppt salinity indicate hyperosmo- and hyperionoregulatory
abilities for more than 1 week, as in crustaceans and teleost fish. While
ventilation frequency decreased by 1 day, Na(+)/K(+)-ATPase activity, which has
been generally implicated in ion transport, was induced in two of the eight
posterior gills after 1 week. In addition, the octopuses were intravenously
injected with 1 or 100 ng/g octopressin or cephalotocin, which are Octopus
vasopressin/oxytocin orthologs. After 1 day, octopressin, but not cephalotocin,
decreased the hemolymph osmolality and Ca concentrations, as well as urinary Na
concentrations. These data provide evidence for possible parallel evolution in
hyperionoregulatory mechanisms and coordination by conserved peptides.