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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Front+Bioeng+Biotechnol
2015 ; 3
(ä): 147
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Fluorescent Reporter Libraries as Useful Tools for Optimizing Microbial Cell
Factories: A Review of the Current Methods and Applications
#MMPMID26442261
Delvigne F
; Pêcheux H
; Tarayre C
Front Bioeng Biotechnol
2015[]; 3
(ä): 147
PMID26442261
show ga
The use of genetically encoded fluorescent reporters allows speeding up the
initial optimization steps of microbial bioprocesses. These reporters can be used
for determining the expression level of a particular promoter, not only the
synthesis of a specific protein but also the content of intracellular
metabolites. The level of protein/metabolite is thus proportional to a
fluorescence signal. By this way, mean expression profiles of protein/metabolites
can be determined non-invasively at a high-throughput rate, allowing the rapid
identification of the best producers. Actually, different kinds of reporter
systems are available, as well as specific cultivation devices allowing the
on-line recording of the fluorescent signal. Cell-to-cell variability is another
important phenomenon that can be integrated into the screening procedures for the
selection of more efficient microbial cell factories.