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10.3389/fbioe.2015.00147

http://scihub22266oqcxt.onion/10.3389/fbioe.2015.00147
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suck abstract from ncbi


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pmid26442261
      Front+Bioeng+Biotechnol 2015 ; 3 (ä): 147
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  • Fluorescent Reporter Libraries as Useful Tools for Optimizing Microbial Cell Factories: A Review of the Current Methods and Applications #MMPMID26442261
  • Delvigne F ; Pêcheux H ; Tarayre C
  • Front Bioeng Biotechnol 2015[]; 3 (ä): 147 PMID26442261 show ga
  • The use of genetically encoded fluorescent reporters allows speeding up the initial optimization steps of microbial bioprocesses. These reporters can be used for determining the expression level of a particular promoter, not only the synthesis of a specific protein but also the content of intracellular metabolites. The level of protein/metabolite is thus proportional to a fluorescence signal. By this way, mean expression profiles of protein/metabolites can be determined non-invasively at a high-throughput rate, allowing the rapid identification of the best producers. Actually, different kinds of reporter systems are available, as well as specific cultivation devices allowing the on-line recording of the fluorescent signal. Cell-to-cell variability is another important phenomenon that can be integrated into the screening procedures for the selection of more efficient microbial cell factories.
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