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Isolation and molecular characterization of nephropathic infectious bronchitis
virus isolates of Gujarat state, India
#MMPMID26436120
Patel BH
; Bhimani MP
; Bhanderi BB
; Jhala MK
Virusdisease
2015[Jun]; 26
(1-2
): 42-7
PMID26436120
show ga
Infectious bronchitis (IB) is a common, highly contagious, acute, and
economically important viral disease of chickens caused by Infectious bronchitis
virus (IBV, sp. Avian coronavirus). Five pooled tissue suspensions of 50 layer
birds and one reference Massachusetts vaccine strain were inoculated into
specific pathogen free (SPF) chicken egg for isolation of IBV.
Reverse-transcription polymerase chain reaction (RT-PCR) was carried out using
post inoculated allontoic fluid to amplify the spike (S) glycoprotein of S1
subunit of IBV. All the eggs inoculated with five pooled tissue samples and
vaccine sample showed dwarfing and curling of SPF embryos indicative of IBV. All
the five samples and the vaccine sample produced the expected amplicons of 466 bp
by RT-PCR. The sequencing of five isolates revealed that all the five sequences
were 99.09-100 % similar among themselves and showed 99.10-100 % nucleotide
identity with the vaccine strain. On multiple sequence alignment it was found
that our isolates were more similar at S1 subunit nucleotide level with the
reference Ma5 and H120 vaccine strains than the reference Mass41 strain. The
sequences of Anand isolates revealed further genetic changes in the circulating
IBV in comparison to previous isolate of Gujarat as well as higher differences
with the strains isolated in other states showing substantial changes at genetic
level in Indian IBV isolates, which may partially explain the increasing
incidences of IB in the country in spite of the vaccination.
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