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10.1016/j.cell.2015.08.046 http://scihub22266oqcxt.onion/10.1016/j.cell.2015.08.046 C4583717!4583717!26406378     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2015 ; 163 (1): 218-29 Nephropedia Template TP {{tp|p=26406378|t=2015. Tracing dynamic changes of DNA methylation at single cell resolution |pdf=|usr=}}{{26406378}} gab.com Text Tracing dynamic changes of DNA methylation at single cell resolution JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=26406378 #FOAvip Mammalian DNA methylation plays an essential role in development. To date, only snapshots of different mouse and human cell types have been generated, providing a static view on DNA methylation. To enable monitoring of methylation status as it changes over time, we establish a Reporter of Genomic Methylation (RGM) that relies on a minimal imprinted gene promoter driving a fluorescent protein. We show that insertion of RGM proximal to promoter-associated CpG islands reports the gain and loss of DNA methylation. We further utilized RGM to report endogenous methylation dynamics of non-coding regulatory elements, such as the pluripotency-specific super enhancers of Sox2 and miR290. Loci-specific DNA methylation changes, and its correlation with transcription was visualized during cell state transition following differentiation of mouse embryonic stem cells and during reprogramming of somatic cells to pluripotency. RGM will allow the investigation of dynamic methylation changes during development and disease at single cell resolution. Twit Text FOAVip Tracing dynamic changes of DNA methylation at single cell resolution ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=26406378 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26406378 #FOAvip English Wikipedia <ref>{{Cite pmid|26406378}}</ref> Tracing dynamic changes of DNA methylation at single cell resolution #MMPMID26406378 Stelzer Y; Shivalila CS; Soldner F; Markoulaki S; Jaenisch R Cell 2015[Sep]; 163 (1): 218-29 PMID26406378show ga Mammalian DNA methylation plays an essential role in development. To date, only snapshots of different mouse and human cell types have been generated, providing a static view on DNA methylation. To enable monitoring of methylation status as it changes over time, we establish a Reporter of Genomic Methylation (RGM) that relies on a minimal imprinted gene promoter driving a fluorescent protein. We show that insertion of RGM proximal to promoter-associated CpG islands reports the gain and loss of DNA methylation. We further utilized RGM to report endogenous methylation dynamics of non-coding regulatory elements, such as the pluripotency-specific super enhancers of Sox2 and miR290. Loci-specific DNA methylation changes, and its correlation with transcription was visualized during cell state transition following differentiation of mouse embryonic stem cells and during reprogramming of somatic cells to pluripotency. RGM will allow the investigation of dynamic methylation changes during development and disease at single cell resolution. äTracing dynamic changes of DNA methylation at single cell resolution (epmc).pdf DeepDyve Pubget Overpricing