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10.1002/elps.201300242 http://scihub22266oqcxt.onion/10.1002/elps.201300242 C4583218!4583218 !23925921     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=23925921 &cmd=llinks): Failed to open stream: HTTP request failed! 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Characterization of a hybrid dielectrophoresis and immunocapture microfluidic system for cancer cell capture |pdf=|usr=}}{{23925921 }} gab.com Text Characterization of a hybrid dielectrophoresis and immunocapture microfluidic system for cancer cell capture JO: Electrophoresis http://www.kidney.de/pget.php?&tw=1&pmid=23925921 #FOAvip The capture of circulating tumor cells (CTCs) from cancer patient blood enables early clinical assessment as well as genetic and pharmacological evaluation of cancer and metastasis. Although there have been many microfluidic immunocapture and electrokinetic techniques developed for isolating rare cancer cells, these techniques are often limited by a capture performance tradeoff between high efficiency and high purity. We present the characterization of shear-dependent cancer cell capture in a novel hybrid DEP-immunocapture system consisting of interdigitated electrodes fabricated in a Hele-Shaw flow cell that was functionalized with a monoclonal antibody, J591, which is highly specific to prostate-specific membrane antigen expressing prostate cancer cells. We measured the positive and negative DEP response of a prostate cancer cell line, LNCaP, as a function of applied electric field frequency, and showed that DEP can control capture performance by promoting or preventing cell interactions with immunocapture surfaces, depending on the sign and magnitude of the applied DEP force, as well as on the local shear stress experienced by cells flowing in the device. This work demonstrates that DEP and immunocapture techniques can work synergistically to improve cell capture performance, and it will aid in the design of future hybrid DEP-immunocapture systems for high-efficiency CTC capture with enhanced purity. Twit Text FOAVip Characterization of a hybrid dielectrophoresis and immunocapture microfluidic system for cancer cell capture ????Electrophoresis http://www.kidney.de/pget.php?&tw=1&pmid=23925921 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23925921 #FOAvip English Wikipedia <ref>{{Cite pmid|23925921 }}</ref> Characterization of a hybrid dielectrophoresis and immunocapture microfluidic system for cancer cell capture #MMPMID23925921 Huang C ; Santana SM ; Liu H ; Bander NH ; Hawkins BG ; Kirby BJ Electrophoresis 2013[Nov]; 34 (20-21 ): 2970-9 PMID23925921 show ga The capture of circulating tumor cells (CTCs) from cancer patient blood enables early clinical assessment as well as genetic and pharmacological evaluation of cancer and metastasis. Although there have been many microfluidic immunocapture and electrokinetic techniques developed for isolating rare cancer cells, these techniques are often limited by a capture performance tradeoff between high efficiency and high purity. We present the characterization of shear-dependent cancer cell capture in a novel hybrid DEP-immunocapture system consisting of interdigitated electrodes fabricated in a Hele-Shaw flow cell that was functionalized with a monoclonal antibody, J591, which is highly specific to prostate-specific membrane antigen expressing prostate cancer cells. We measured the positive and negative DEP response of a prostate cancer cell line, LNCaP, as a function of applied electric field frequency, and showed that DEP can control capture performance by promoting or preventing cell interactions with immunocapture surfaces, depending on the sign and magnitude of the applied DEP force, as well as on the local shear stress experienced by cells flowing in the device. This work demonstrates that DEP and immunocapture techniques can work synergistically to improve cell capture performance, and it will aid in the design of future hybrid DEP-immunocapture systems for high-efficiency CTC capture with enhanced purity. |Antibodies, Immobilized/chemistry [MESH] |Antibodies, Monoclonal/chemistry [MESH] |Cell Line, Tumor [MESH] |Cell Separation/*instrumentation [MESH] |Electrodes [MESH] |Equipment Design [MESH] |Humans [MESH] |Male [MESH] |Microfluidic Analytical Techniques/*instrumentation [MESH] |Neoplastic Cells, Circulating/*pathology [MESH]Characterization of a hybrid dielectrophoresis and immunocapture micro(epmc).pdf DeepDyve Pubget Overpricing