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10.3390/ijms160816897 http://scihub22266oqcxt.onion/10.3390/ijms160816897 C4581176!4581176!26213926     free     free     free       Int+J+Mol+Sci 2015 ; 16 (8): 16897-919 Nephropedia Template TP {{tp|p=26213926|t=2015. Technologies for Single-Cell Isolation |pdf=|usr=}}{{26213926}} gab.com Text Technologies for Single-Cell Isolation JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=26213926 #FOAvip The handling of single cells is of great importance in applications such as cell line development or single-cell analysis, e.g., for cancer research or for emerging diagnostic methods. This review provides an overview of technologies that are currently used or in development to isolate single cells for subsequent single-cell analysis. Data from a dedicated online market survey conducted to identify the most relevant technologies, presented here for the first time, shows that FACS (fluorescence activated cell sorting) respectively Flow cytometry (33% usage), laser microdissection (17%), manual cell picking (17%), random seeding/dilution (15%), and microfluidics/lab-on-a-chip devices (12%) are currently the most frequently used technologies. These most prominent technologies are described in detail and key performance factors are discussed. The survey data indicates a further increasing interest in single-cell isolation tools for the coming years. Additionally, a worldwide patent search was performed to screen for emerging technologies that might become relevant in the future. In total 179 patents were found, out of which 25 were evaluated by screening the title and abstract to be relevant to the field. Twit Text FOAVip Technologies for Single-Cell Isolation ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=26213926 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26213926 #FOAvip English Wikipedia <ref>{{Cite pmid|26213926}}</ref> Technologies for Single-Cell Isolation #MMPMID26213926 Gross A; Schoendube J; Zimmermann S; Steeb M; Zengerle R; Koltay P Int J Mol Sci 2015[Aug]; 16 (8): 16897-919 PMID26213926show ga The handling of single cells is of great importance in applications such as cell line development or single-cell analysis, e.g., for cancer research or for emerging diagnostic methods. This review provides an overview of technologies that are currently used or in development to isolate single cells for subsequent single-cell analysis. Data from a dedicated online market survey conducted to identify the most relevant technologies, presented here for the first time, shows that FACS (fluorescence activated cell sorting) respectively Flow cytometry (33% usage), laser microdissection (17%), manual cell picking (17%), random seeding/dilution (15%), and microfluidics/lab-on-a-chip devices (12%) are currently the most frequently used technologies. These most prominent technologies are described in detail and key performance factors are discussed. The survey data indicates a further increasing interest in single-cell isolation tools for the coming years. Additionally, a worldwide patent search was performed to screen for emerging technologies that might become relevant in the future. In total 179 patents were found, out of which 25 were evaluated by screening the title and abstract to be relevant to the field. äTechnologies for Single-Cell Isolation (epmc).pdf DeepDyve Pubget Overpricing