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2015 ; 169
(1
): 485-96
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Pollen Tube Discharge Completes the Process of Synergid Degeneration That Is
Initiated by Pollen Tube-Synergid Interaction in Arabidopsis
#MMPMID26229050
Leydon AR
; Tsukamoto T
; Dunatunga D
; Qin Y
; Johnson MA
; Palanivelu R
Plant Physiol
2015[Sep]; 169
(1
): 485-96
PMID26229050
show ga
In flowering plant reproduction, pollen tube reception is the signaling system
that results in pollen tube discharge, synergid degeneration, and successful
delivery of male gametes (two sperm cells) to the site where they can fuse with
female gametes (egg cell and central cell). Some molecules required for this
complex and essential signaling exchange have been identified; however,
fundamental questions about the nature of the interactions between the pollen
tube and the synergid cells remain to be clarified. Here, we monitor pollen tube
arrival, pollen tube discharge, and synergid degeneration in Arabidopsis
(Arabidopsis thaliana) wild type and in male and female gametophytic mutants that
disrupt development and function of the gametophytes. By combining assays used
previously to study these interactions and an assay that facilitates simultaneous
analysis of pollen tube discharge and synergid degeneration, we find that
synergid degeneration could be initiated without pollen tube discharge. Our data
support the hypothesis that pollen tube-synergid contact, or signaling via
secreted molecules, initiates receptive synergid degeneration. We also find that
when pollen tubes successfully burst, they always discharge into a degenerated
synergid. In addition to this pollen tube-dependent promotion of synergid
degeneration, we also show that a basal developmental pathway mediates synergid
degeneration in the absence of pollination. Our results are consistent with the
model that a complex set of interactions between the pollen tube and synergid
cells promote receptive synergid degeneration.
|Arabidopsis/*cytology
[MESH]
|Mutation/genetics
[MESH]
|Ovule/*cytology
[MESH]
|Pollen Tube/*cytology/growth & development
[MESH]