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10.1038/nature14887 http://scihub22266oqcxt.onion/10.1038/nature14887 C4575634!4575634!26322582     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nature 2015 ; 525 (7569): 345-50 Nephropedia Template TP {{tp|p=26322582|t=2015. The mechanism of DNA replication termination in vertebrates |pdf=|usr=}}{{26322582}} gab.com Text The mechanism of DNA replication termination in vertebrates JO: Nature http://www.kidney.de/pget.php?&tw=1&pmid=26322582 #FOAvip Eukaryotic DNA replication terminates when replisomes from adjacent replication origins converge. Termination involves local completion of DNA synthesis, decatenation of daughter molecules, and replisome disassembly. Termination has been difficult to study because termination events are generally asynchronous and sequence non-specific. To overcome these challenges, we paused converging replisomes with a site-specific barrier in Xenopus egg extracts. Upon removal of the barrier, forks underwent synchronous and site-specific termination, allowing mechanistic dissection of this process. We show that DNA synthesis does not slow detectably as forks approach each other and that leading strands pass each other unhindered before undergoing ligation to downstream lagging strands. Dissociation of CMG helicases occurs only after the final ligation step, and is not required for completion of DNA synthesis, strongly suggesting that converging CMGs pass one another and dissociate from double-stranded DNA. This termination mechanism allows rapid completion of DNA synthesis while avoiding premature replisome disassembly Twit Text FOAVip The mechanism of DNA replication termination in vertebrates ????Nature http://www.kidney.de/pget.php?&tw=1&pmid=26322582 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26322582 #FOAvip English Wikipedia <ref>{{Cite pmid|26322582}}</ref> The mechanism of DNA replication termination in vertebrates #MMPMID26322582 Dewar JM; Budzowska M; Walter JC Nature 2015[Sep]; 525 (7569): 345-50 PMID26322582show ga Eukaryotic DNA replication terminates when replisomes from adjacent replication origins converge. Termination involves local completion of DNA synthesis, decatenation of daughter molecules, and replisome disassembly. Termination has been difficult to study because termination events are generally asynchronous and sequence non-specific. To overcome these challenges, we paused converging replisomes with a site-specific barrier in Xenopus egg extracts. Upon removal of the barrier, forks underwent synchronous and site-specific termination, allowing mechanistic dissection of this process. We show that DNA synthesis does not slow detectably as forks approach each other and that leading strands pass each other unhindered before undergoing ligation to downstream lagging strands. Dissociation of CMG helicases occurs only after the final ligation step, and is not required for completion of DNA synthesis, strongly suggesting that converging CMGs pass one another and dissociate from double-stranded DNA. This termination mechanism allows rapid completion of DNA synthesis while avoiding premature replisome disassembly äThe mechanism of DNA replication termination in vertebrates (epmc).pdf DeepDyve Pubget Overpricing