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10.1016/j.mito.2015.07.001 http://scihub22266oqcxt.onion/10.1016/j.mito.2015.07.001 C4570932!4570932 !26184437     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26184437 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mitochondrion 2015 ; 24 (ä): 77-86 Nephropedia Template TP {{tp|p=26184437 |t=2015. MitoLoc: A method for the simultaneous quantification of mitochondrial network morphology and membrane potential in single cells |pdf=|usr=}}{{26184437 }} gab.com Text MitoLoc: A method for the simultaneous quantification of mitochondrial network morphology and membrane potential in single cells JO: Mitochondrion http://www.kidney.de/pget.php?&tw=1&pmid=26184437 #FOAvip Mitochondria assemble into flexible networks. Here we present a simple method for the simultaneous quantification of mitochondrial membrane potential and network morphology that is based on computational co-localisation analysis of differentially imported fluorescent marker proteins. Established in, but not restricted to, Saccharomyces cerevisiae, MitoLoc reproducibly measures changes in membrane potential induced by the uncoupling agent CCCP, by oxidative stress, in respiratory deficient cells, and in ?fzo1, ?ref2, and ?dnm1 mutants that possess fission and fusion defects. In combination with super-resolution images, MitoLoc uses 3D reconstruction to calculate six geometrical classifiers which differentiate network morphologies in ?fzo1, ?ref2, and ?dnm1 mutants, under oxidative stress and in cells lacking mtDNA, even when the network is fragmented to a similar extent. We find that mitochondrial fission and a decline in membrane potential do regularly, but not necessarily, co-occur. MitoLoc hence simplifies the measurement of mitochondrial membrane potential in parallel to detect morphological changes in mitochondrial networks. Marker plasmid open-source software as well as the mathematical procedures are made openly available. Twit Text FOAVip MitoLoc: A method for the simultaneous quantification of mitochondrial network morphology and membrane potential in single cells ????Mitochondrion http://www.kidney.de/pget.php?&tw=1&pmid=26184437 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26184437 #FOAvip English Wikipedia <ref>{{Cite pmid|26184437 }}</ref> MitoLoc: A method for the simultaneous quantification of mitochondrial network morphology and membrane potential in single cells #MMPMID26184437 Vowinckel J ; Hartl J ; Butler R ; Ralser M Mitochondrion 2015[Sep]; 24 (ä): 77-86 PMID26184437 show ga Mitochondria assemble into flexible networks. Here we present a simple method for the simultaneous quantification of mitochondrial membrane potential and network morphology that is based on computational co-localisation analysis of differentially imported fluorescent marker proteins. Established in, but not restricted to, Saccharomyces cerevisiae, MitoLoc reproducibly measures changes in membrane potential induced by the uncoupling agent CCCP, by oxidative stress, in respiratory deficient cells, and in ?fzo1, ?ref2, and ?dnm1 mutants that possess fission and fusion defects. In combination with super-resolution images, MitoLoc uses 3D reconstruction to calculate six geometrical classifiers which differentiate network morphologies in ?fzo1, ?ref2, and ?dnm1 mutants, under oxidative stress and in cells lacking mtDNA, even when the network is fragmented to a similar extent. We find that mitochondrial fission and a decline in membrane potential do regularly, but not necessarily, co-occur. MitoLoc hence simplifies the measurement of mitochondrial membrane potential in parallel to detect morphological changes in mitochondrial networks. Marker plasmid open-source software as well as the mathematical procedures are made openly available. |*Membrane Potentials [MESH] |Biometry [MESH] |Genes, Reporter [MESH] |Imaging, Three-Dimensional [MESH] |Microscopy, Fluorescence [MESH] |Mitochondria/*physiology/*ultrastructure [MESH] |Models, Theoretical [MESH] |Single-Cell Analysis [MESH]MitoLoc: A method for the simultaneous quantification of mitochondrial(epmc).pdf DeepDyve Pubget Overpricing