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2015 ; 15
(8
): 20511-23
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Considerations on Circuit Design and Data Acquisition of a Portable Surface
Plasmon Resonance Biosensing System
#MMPMID26295398
Chang K
; Chen R
; Wang S
; Li J
; Hu X
; Liang H
; Cao B
; Sun X
; Ma L
; Zhu J
; Jiang M
; Hu J
Sensors (Basel)
2015[Aug]; 15
(8
): 20511-23
PMID26295398
show ga
The aim of this study was to develop a circuit for an inexpensive portable
biosensing system based on surface plasmon resonance spectroscopy. This portable
biosensing system designed for field use is characterized by a special structure
which consists of a microfluidic cell incorporating a right angle prism
functionalized with a biomolecular identification membrane, a laser line
generator and a data acquisition circuit board. The data structure, data memory
capacity and a line charge-coupled device (CCD) array with a driving circuit for
collecting the photoelectric signals are intensively focused on and the high
performance analog-to-digital (A/D) converter is comprehensively evaluated. The
interface circuit and the photoelectric signal amplifier circuit are first
studied to obtain the weak signals from the line CCD array in this experiment.
Quantitative measurements for validating the sensitivity of the biosensing system
were implemented using ethanol solutions of various concentrations indicated by
volume fractions of 5%, 8%, 15%, 20%, 25%, and 30%, respectively, without a
biomembrane immobilized on the surface of the SPR sensor. The experiments
demonstrated that it is possible to detect a change in the refractive index of an
ethanol solution with a sensitivity of 4.99838 × 10(5) ?RU/RI in terms of the
changes in delta response unit with refractive index using this SPR biosensing
system, whereby the theoretical limit of detection of 3.3537 × 10(-5) refractive
index unit (RIU) and a high linearity at the correlation coefficient of 0.98065.
The results obtained from a series of tests confirmed the practicality of this
cost-effective portable SPR biosensing system.