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2015 ; 24
(5
): 921-7
Nephropedia Template TP
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English Wikipedia
Blastocyst genotyping for quality control of mouse mutant archives: an ethical
and economical approach
#MMPMID26178246
Scavizzi F
; Ryder E
; Newman S
; Raspa M
; Gleeson D
; Wardle-Jones H
; Montoliu L
; Fernandez A
; Dessain ML
; Larrigaldie V
; Khorshidi Z
; Vuolteenaho R
; Soininen R
; André P
; Jacquot S
; Hong Y
; de Angelis MH
; Ramirez-Solis R
; Doe B
Transgenic Res
2015[Oct]; 24
(5
): 921-7
PMID26178246
show ga
With the advent of modern developmental biology and molecular genetics, the
scientific community has generated thousands of newly genetically altered strains
of laboratory mice with the aim of elucidating gene function. To this end, a
large group of Institutions which form the International Mouse Phenotyping
Consortium is generating and phenotyping a knockout mouse strain for each of the
~20,000 protein-coding genes using the mutant ES cell resource produced by the
International Knockout Mouse Consortium. These strains are made available to the
research community via public repositories, mostly as cryopreserved sperm or
embryos. To ensure the quality of this frozen resource there is a requirement
that for each strain the frozen sperm/embryos are proven able to produce viable
mutant progeny, before the live animal resource is removed from cages. Given the
current requirement to generate live pups to demonstrate their mutant genotype,
this quality control check necessitates the use and generation of many animals
and requires considerable time, cage space, technical and economic resources.
Here, we describe a simple and efficient method of genotyping pre-implantation
stage blastocysts with significant ethical and economic advantages especially
beneficial for current and future large-scale mouse mutagenesis projects.