Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1371/journal.pone.0137711 http://scihub22266oqcxt.onion/10.1371/journal.pone.0137711 C4569406!4569406!26368822     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2015 ; 10 (9): ä Nephropedia Template TP {{tp|p=26368822|t=2015. Identification of Tetraazacyclic Compounds as Novel Potent Inhibitors Antagonizing ROR?t Activity and Suppressing Th17 Cell Differentiation |pdf=|usr=}}{{26368822}} gab.com Text Identification of Tetraazacyclic Compounds as Novel Potent Inhibitors Antagonizing ROR t Activity and Suppressing Th17 Cell Differentiation JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26368822 #FOAvip CD4+ T-helper cells that produce interleukin-17 (Th17 cells) are characterized as pathological T-helper cells in autoimmune diseases. Differentiation of human and mouse Th17 cells requires a key transcription regulator, retinoic acid receptor-related orphan receptor ?t (ROR?t), which is a potential therapeutic target for autoimmune diseases. To develop a therapeutic agent for Th17-mediated autoimmune diseases, we have established a high-throughput screening (HTS) assay for candidate screening, in which the luciferase activity in ROR?t-LBD positive and negative Jurkat cells were analyzed to evaluate induction of ROR?t activity by compounds. This technique was applied to screen a commercially-available drug-like chemical compound library (Enamine) which contains 20155 compounds. The screening identified 17 compounds that can inhibit ROR?t function in the HTS screen system. Of these, three tetraazacyclic compounds can potently inhibit ROR?t activity, and suppress Th17 differentiation and IL-17 production. These three candidate compounds could significantly attenuate the expression of the Il17a by 65%- 90%, and inhibit IL-17A secretion by 47%, 63%, and 74%, respectively. These compounds also exhibited a potent anti-ROR?t activity, with EC50 values of 0.25 ?M, 0.67 ?M and 2.6 ?M, respectively. Our data demonstrated the feasibility of targeting the ROR?t to inhibit Th17 cell differentiation and function with these tetraazacyclic compounds, and the potential to improve the structure of these compounds for autoimmune diseases therapeutics. Twit Text FOAVip Identification of Tetraazacyclic Compounds as Novel Potent Inhibitors Antagonizing ROR t Activity and Suppressing Th17 Cell Differentiation ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26368822 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26368822 #FOAvip English Wikipedia <ref>{{Cite pmid|26368822}}</ref> Identification of Tetraazacyclic Compounds as Novel Potent Inhibitors Antagonizing ROR?t Activity and Suppressing Th17 Cell Differentiation #MMPMID26368822 Ding Q; Zhao M; Yu B; Bai C; Huang Z PLoS One 2015[]; 10 (9): ä PMID26368822show ga CD4+ T-helper cells that produce interleukin-17 (Th17 cells) are characterized as pathological T-helper cells in autoimmune diseases. Differentiation of human and mouse Th17 cells requires a key transcription regulator, retinoic acid receptor-related orphan receptor ?t (ROR?t), which is a potential therapeutic target for autoimmune diseases. To develop a therapeutic agent for Th17-mediated autoimmune diseases, we have established a high-throughput screening (HTS) assay for candidate screening, in which the luciferase activity in ROR?t-LBD positive and negative Jurkat cells were analyzed to evaluate induction of ROR?t activity by compounds. This technique was applied to screen a commercially-available drug-like chemical compound library (Enamine) which contains 20155 compounds. The screening identified 17 compounds that can inhibit ROR?t function in the HTS screen system. Of these, three tetraazacyclic compounds can potently inhibit ROR?t activity, and suppress Th17 differentiation and IL-17 production. These three candidate compounds could significantly attenuate the expression of the Il17a by 65%- 90%, and inhibit IL-17A secretion by 47%, 63%, and 74%, respectively. These compounds also exhibited a potent anti-ROR?t activity, with EC50 values of 0.25 ?M, 0.67 ?M and 2.6 ?M, respectively. Our data demonstrated the feasibility of targeting the ROR?t to inhibit Th17 cell differentiation and function with these tetraazacyclic compounds, and the potential to improve the structure of these compounds for autoimmune diseases therapeutics. äIdentification of Tetraazacyclic Compounds as Novel Potent Inhibitors (epmc).pdf DeepDyve Pubget Overpricing