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2015 ; 37
(3
): 505-14
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Salivary cortisol results obtainable within minutes of sample collection
correspond with traditional immunoassays
#MMPMID25773457
Shirtcliff EA
; Buck RL
; Laughlin MJ
; Hart T
; Cole CR
; Slowey PD
Clin Ther
2015[Mar]; 37
(3
): 505-14
PMID25773457
show ga
PURPOSE: Cortisol is frequently assayed as a stress-responsive biomarker which
changes over the course of minutes to meet the demands of a person's social
context. Salivary cortisol is often used as a noninvasive sampling method that
possesses important health implications. A critical barrier to psychobiological
research that involves salivary cortisol is a time delay of days to months before
cortisol results are obtained via immunoassay, long after the person is no longer
proximate to the social context in which they provided the sample. The present
study was designed to address this critical barrier through creation of a lateral
flow test (LFT) cortisol device capable of measuring salivary cortisol within
minutes of sample collection. The LFT is frequently used within commercial
point-of-care settings to obtain rapid answers to the presence/absence of a
biomarker. The present study extends the LFT into the research domain by
presenting performance characteristics of a quantitative LFT that measures
salivary cortisol within 20 minutes of sample collection. METHODS: Saliva samples
from 29 adults (15 men) were obtained in the morning and afternoon by using
Passive Drool and then the Super·SAL Extra Collection Device (hereafter
Super·SAL) and later assayed with LFT and a commercially available enzyme
immunoassay. FINDINGS: Results indicate the LFT correlated well with these
collection methods (R = 0.872 with Super · SAL, R = 0.739 with Passive Drool, P <
0.0001) and at comparable levels to correspondence of Super · SAL with Passive
Drool (R = 0.798, P < 0.0001) which were measured with the same assay.
IMPLICATIONS: These results open an exciting new possibility to integrate this
technologic advance into stress research, including knowing and potentially
changing the person's social context in a time-sensitive manner. Methodological
improvements such as this have the possibility of refining conceptual models of
stress reactivity and regulation.