Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1007/s10787-015-0247-y http://scihub22266oqcxt.onion/10.1007/s10787-015-0247-y C4568011!4568011!26254778     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Inflammopharmacology 2015 ; 23 (5): 283-90 Nephropedia Template TP {{tp|p=26254778|t=2015. T-bet and interleukin-27: possible TH1 immunomodulators of sarcoidosis |pdf=|usr=}}{{26254778}} gab.com Text T-bet and interleukin-27: possible TH1 immunomodulators of sarcoidosis JO: Inflammopharmacology http://www.kidney.de/pget.php?&tw=1&pmid=26254778 #FOAvip Background: Sarcoidosis has often been termed an ?immune paradox? as there is peripheral anergy to common recall antigens despite pronounced TH1-dominant inflammation at disease sites, such as the lung, with up-regulation of interferon ?, IL-27 and transcription factors. Peripheral blood may reflect the anergic state, while exhaled breath condensate (EBC) analysis may offer insights into the lung disease. Methods: A cross-sectional study was conducted to investigate the expression of TH1 cytokines and transcription factors (IFN?, IL-27 and T-bet) in the peripheral blood and/or EBC of sarcoidosis patients and healthy controls. Whole blood and EBC were collected from sarcoidosis patients and healthy controls. TH1 cytokine expression levels were then measured in peripheral blood mononuclear cells (PBMCs) and/or plasma and EBC using quantitative real-time PCR, ELISA and via Western blotting. Results: Compared to healthy controls, PBMC IL-27 mRNA was higher in patients (p = 0.0019). There were no significant differences in plasma IL-27 protein between patients and controls (p = 0.20). T-bet mRNA and protein were lower (p = 0.010 and p = 0.0043, respectively) in patients compared to controls. There were no significant differences in PBMC IFN? mRNA and protein expression (p = 0.68 and p = 0.74, respectively) nor in EBC IL-27 levels. Conclusions: Our data indicate that depressed T-bet mRNA and protein expression could contribute to the peripheral anergy in sarcoidosis and that IL-27 mRNA levels are elevated in the PBMC from those with sarcoidosis. Electronic supplementary material: The online version of this article (doi:10.1007/s10787-015-0247-y) contains supplementary material, which is available to authorised users. Twit Text FOAVip T-bet and interleukin-27: possible TH1 immunomodulators of sarcoidosis ????Inflammopharmacology http://www.kidney.de/pget.php?&tw=1&pmid=26254778 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26254778 #FOAvip English Wikipedia <ref>{{Cite pmid|26254778}}</ref> T-bet and interleukin-27: possible TH1 immunomodulators of sarcoidosis #MMPMID26254778 Loke WSJ; Freeman A; Garthwaite L; Prazakova S; Park M; Hsu K; Thomas PS; Herbert C Inflammopharmacology 2015[]; 23 (5): 283-90 PMID26254778show ga Background: Sarcoidosis has often been termed an ?immune paradox? as there is peripheral anergy to common recall antigens despite pronounced TH1-dominant inflammation at disease sites, such as the lung, with up-regulation of interferon ?, IL-27 and transcription factors. Peripheral blood may reflect the anergic state, while exhaled breath condensate (EBC) analysis may offer insights into the lung disease. Methods: A cross-sectional study was conducted to investigate the expression of TH1 cytokines and transcription factors (IFN?, IL-27 and T-bet) in the peripheral blood and/or EBC of sarcoidosis patients and healthy controls. Whole blood and EBC were collected from sarcoidosis patients and healthy controls. TH1 cytokine expression levels were then measured in peripheral blood mononuclear cells (PBMCs) and/or plasma and EBC using quantitative real-time PCR, ELISA and via Western blotting. Results: Compared to healthy controls, PBMC IL-27 mRNA was higher in patients (p = 0.0019). There were no significant differences in plasma IL-27 protein between patients and controls (p = 0.20). T-bet mRNA and protein were lower (p = 0.010 and p = 0.0043, respectively) in patients compared to controls. There were no significant differences in PBMC IFN? mRNA and protein expression (p = 0.68 and p = 0.74, respectively) nor in EBC IL-27 levels. Conclusions: Our data indicate that depressed T-bet mRNA and protein expression could contribute to the peripheral anergy in sarcoidosis and that IL-27 mRNA levels are elevated in the PBMC from those with sarcoidosis. Electronic supplementary material: The online version of this article (doi:10.1007/s10787-015-0247-y) contains supplementary material, which is available to authorised users. äT-bet and interleukin-27: possible TH1 immunomodulators of sarcoidosis(epmc).pdf DeepDyve Pubget Overpricing