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10.1016/j.celrep.2015.02.031 http://scihub22266oqcxt.onion/10.1016/j.celrep.2015.02.031 C4567530!4567530!25772363     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep 2015 ; 10 (10): 1767-77 Nephropedia Template TP {{tp|p=25772363|t=2015. Dynamic Visualization of mTORC1 Activity in Living Cells |pdf=|usr=}}{{25772363}} gab.com Text Dynamic Visualization of mTORC1 Activity in Living Cells JO: Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=25772363 #FOAvip The mechanistic target of rapamycin complex 1 (mTORC1) senses diverse signals to regulate cell growth and metabolism. It has become increasingly clear that mTORC1 activity is regulated in time and space inside the cell, but direct interrogation of such spatiotemporal regulation is challenging. Here we describe a genetically encoded mTORC1 activity reporter (TORCAR) that exhibits a change in FRET in response to phosphorylation by mTORC1. Co-imaging mTORC1 activity and calcium dynamics revealed that a growth factor-induced calcium transient contributes to mTORC1 activity. Dynamic activity maps generated using subcellularly targeted TORCAR uncovered mTORC1 activity not only in cytosol and at the lysosome but also in nucleus and at the plasma membrane. Furthermore, a wide distribution of activities was observed upon growth factor stimulation, whereas leucine ester, an amino acid surrogate, induces more compartmentalized activities at the lysosome and in nucleus. Thus, mTORC1 activities are spatiotemporally regulated in a signal-specific manner. Twit Text FOAVip Dynamic Visualization of mTORC1 Activity in Living Cells ????Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=25772363 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25772363 #FOAvip English Wikipedia <ref>{{Cite pmid|25772363}}</ref> Dynamic Visualization of mTORC1 Activity in Living Cells #MMPMID25772363 Zhou X; Clister TL; Lowry PR; Seldin MM; Wong GW; Zhang J Cell Rep 2015[Mar]; 10 (10): 1767-77 PMID25772363show ga The mechanistic target of rapamycin complex 1 (mTORC1) senses diverse signals to regulate cell growth and metabolism. It has become increasingly clear that mTORC1 activity is regulated in time and space inside the cell, but direct interrogation of such spatiotemporal regulation is challenging. Here we describe a genetically encoded mTORC1 activity reporter (TORCAR) that exhibits a change in FRET in response to phosphorylation by mTORC1. Co-imaging mTORC1 activity and calcium dynamics revealed that a growth factor-induced calcium transient contributes to mTORC1 activity. Dynamic activity maps generated using subcellularly targeted TORCAR uncovered mTORC1 activity not only in cytosol and at the lysosome but also in nucleus and at the plasma membrane. Furthermore, a wide distribution of activities was observed upon growth factor stimulation, whereas leucine ester, an amino acid surrogate, induces more compartmentalized activities at the lysosome and in nucleus. Thus, mTORC1 activities are spatiotemporally regulated in a signal-specific manner. äDynamic Visualization of mTORC1 Activity in Living Cells (epmc).pdf DeepDyve Pubget Overpricing