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10.1371/journal.pone.0137652 http://scihub22266oqcxt.onion/10.1371/journal.pone.0137652 C4567319!4567319!26360249     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2015 ; 10 (9): ä Nephropedia Template TP {{tp|p=26360249|t=2015. AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach |pdf=|usr=}}{{26360249}} gab.com Text AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26360249 #FOAvip Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (advanced quick assembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability and versatility of AQUA Cloning in selected proof-of-principle applications including targeted insertion-, deletion- and site-directed point-mutagenesis, and combinatorial cloning. Furthermore, we show the one pot de novo assembly of multiple DNA fragments into a single circular plasmid encoding a complex light- and chemically-regulated Boolean A NIMPLY B logic operation. AQUA Cloning harnesses intrinsic in vivo processing of linear DNA fragments with short regions of homology of 16 to 32 bp mediated by Escherichia coli. It does not require any kits, enzymes or preparations of reagents and is the simplest assembly cloning protocol to date. Twit Text FOAVip AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26360249 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26360249 #FOAvip English Wikipedia <ref>{{Cite pmid|26360249}}</ref> AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach #MMPMID26360249 Beyer HM; Gonschorek P; Samodelov SL; Meier M; Weber W; Zurbriggen MD PLoS One 2015[]; 10 (9): ä PMID26360249show ga Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (advanced quick assembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability and versatility of AQUA Cloning in selected proof-of-principle applications including targeted insertion-, deletion- and site-directed point-mutagenesis, and combinatorial cloning. Furthermore, we show the one pot de novo assembly of multiple DNA fragments into a single circular plasmid encoding a complex light- and chemically-regulated Boolean A NIMPLY B logic operation. AQUA Cloning harnesses intrinsic in vivo processing of linear DNA fragments with short regions of homology of 16 to 32 bp mediated by Escherichia coli. It does not require any kits, enzymes or preparations of reagents and is the simplest assembly cloning protocol to date. äAQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach (epmc).pdf DeepDyve Pubget Overpricing