Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

http://scihub22266oqcxt.onion/ C4567034!4567034!26242276     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26242276.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Clin+Exp+Rheumatol 2015 ; 33 (0 91): 123-6 Nephropedia Template TP {{tp|p=26242276|t=2015. Pilot Study to Determine Whether Transient Receptor Potential Melastatin Type 8 (TRPM8) Antibodies Are Detected in Scleroderma |pdf=|usr=}}{{26242276}} gab.com Text Pilot Study to Determine Whether Transient Receptor Potential Melastatin Type 8 (TRPM8) Antibodies Are Detected in Scleroderma JO: Clin Exp Rheumatol http://www.kidney.de/pget.php?&tw=1&pmid=26242276 #FOAvip Objectives: A key mediator in cold-sensation is the protein transient receptor potential melastatin 8 (TRPM8), which is expressed on sensory nerves and cutaneous blood vessels. These receptors are activated by cold temperatures and play a key role in body thermoregulation. Cold sensitivity and Raynaud's phenomenon are frequent clinical features in scleroderma, and are thought to be secondary to a local defect in cutaneous thermoregulation. We investigated whether autoantibodies targeting TRPM8 were present in the sera of patients with scleroderma as evidence for a possible mechanism for an acquired immune mediated defect in thermoregulation. Methods: Sera from 50 well-characterized scleroderma patients with Raynaud's phenomenon were studied. TRPM8 autoantibodies were assayed as follows: (1) immunoprecipitation with 35S-methionine-labeled TRPM8 generated by in vitro transcription and translation, (2) immunoblotting lysates made from cells transiently transfected with TRPM8 cDNA, (3) immunoprecipitation of TRPM8 transfected lysates with detection by blotting and (4) flow cytometry. Results: Fifty scleroderma patients with Raynaud's phenomenon (41 female, 39 Caucasian, 23 with limited scleroderma, and 19 with history of cancer) were studied. Four different methods to assay for TRPM8 antibodies were set up, optimized and validated using commercial antibodies. All 50 scleroderma patients' sera were assayed using each of the above methods, and all were negative for TRPM8 autoantibodies. Conclusions: Antibodies against TRPM8 are not found in scleroderma patient sera, suggesting that the abnormal cold sensitivity and associated abnormal vascular reactivity in scleroderma patients is not the result of an immune process targeting TRPM8. Twit Text FOAVip Pilot Study to Determine Whether Transient Receptor Potential Melastatin Type 8 (TRPM8) Antibodies Are Detected in Scleroderma ????Clin Exp Rheumatol http://www.kidney.de/pget.php?&tw=1&pmid=26242276 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26242276 #FOAvip English Wikipedia <ref>{{Cite pmid|26242276}}</ref> Pilot Study to Determine Whether Transient Receptor Potential Melastatin Type 8 (TRPM8) Antibodies Are Detected in Scleroderma #MMPMID26242276 Shah AA; Montagne J; Oh SY; Wigley FM; Casciola-Rosen L Clin Exp Rheumatol 2015[Jul]; 33 (0 91): 123-6 PMID26242276show ga Objectives: A key mediator in cold-sensation is the protein transient receptor potential melastatin 8 (TRPM8), which is expressed on sensory nerves and cutaneous blood vessels. These receptors are activated by cold temperatures and play a key role in body thermoregulation. Cold sensitivity and Raynaud's phenomenon are frequent clinical features in scleroderma, and are thought to be secondary to a local defect in cutaneous thermoregulation. We investigated whether autoantibodies targeting TRPM8 were present in the sera of patients with scleroderma as evidence for a possible mechanism for an acquired immune mediated defect in thermoregulation. Methods: Sera from 50 well-characterized scleroderma patients with Raynaud's phenomenon were studied. TRPM8 autoantibodies were assayed as follows: (1) immunoprecipitation with 35S-methionine-labeled TRPM8 generated by in vitro transcription and translation, (2) immunoblotting lysates made from cells transiently transfected with TRPM8 cDNA, (3) immunoprecipitation of TRPM8 transfected lysates with detection by blotting and (4) flow cytometry. Results: Fifty scleroderma patients with Raynaud's phenomenon (41 female, 39 Caucasian, 23 with limited scleroderma, and 19 with history of cancer) were studied. Four different methods to assay for TRPM8 antibodies were set up, optimized and validated using commercial antibodies. All 50 scleroderma patients' sera were assayed using each of the above methods, and all were negative for TRPM8 autoantibodies. Conclusions: Antibodies against TRPM8 are not found in scleroderma patient sera, suggesting that the abnormal cold sensitivity and associated abnormal vascular reactivity in scleroderma patients is not the result of an immune process targeting TRPM8. äPilot Study to Determine Whether Transient Receptor Potential Melastat(epmc).pdf DeepDyve Pubget Overpricing