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10.1038/srep14017 http://scihub22266oqcxt.onion/10.1038/srep14017 C4566134!4566134 !26360462     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26360462 &cmd=llinks): Failed to open stream: HTTP request failed! 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An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography |pdf=|usr=}}{{26360462 }} gab.com Text An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26360462 #FOAvip Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that are not amenable to producing large well diffracting crystals. Efficient de novo phasing methods are highly demanding and as such most SFX structures have been determined by molecular replacement methods. Here we employed single isomorphous replacement with anomalous scattering (SIRAS) for phasing and demonstrate successful application to SFX de novo phasing. Only about 20,000 patterns in total were needed for SIRAS phasing while single wavelength anomalous dispersion (SAD) phasing was unsuccessful with more than 80,000 patterns of derivative crystals. We employed high energy X-rays from SACLA (12.6?keV) to take advantage of the large anomalous enhancement near the LIII absorption edge of Hg, which is one of the most widely used heavy atoms for phasing in conventional protein crystallography. Hard XFEL is of benefit for de novo phasing in the use of routinely used heavy atoms and high resolution data collection. Twit Text FOAVip An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26360462 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26360462 #FOAvip English Wikipedia <ref>{{Cite pmid|26360462 }}</ref> An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography #MMPMID26360462 Yamashita K ; Pan D ; Okuda T ; Sugahara M ; Kodan A ; Yamaguchi T ; Murai T ; Gomi K ; Kajiyama N ; Mizohata E ; Suzuki M ; Nango E ; Tono K ; Joti Y ; Kameshima T ; Park J ; Song C ; Hatsui T ; Yabashi M ; Iwata S ; Kato H ; Ago H ; Yamamoto M ; Nakatsu T Sci Rep 2015[Sep]; 5 (ä): 14017 PMID26360462 show ga Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that are not amenable to producing large well diffracting crystals. Efficient de novo phasing methods are highly demanding and as such most SFX structures have been determined by molecular replacement methods. Here we employed single isomorphous replacement with anomalous scattering (SIRAS) for phasing and demonstrate successful application to SFX de novo phasing. Only about 20,000 patterns in total were needed for SIRAS phasing while single wavelength anomalous dispersion (SAD) phasing was unsuccessful with more than 80,000 patterns of derivative crystals. We employed high energy X-rays from SACLA (12.6?keV) to take advantage of the large anomalous enhancement near the LIII absorption edge of Hg, which is one of the most widely used heavy atoms for phasing in conventional protein crystallography. Hard XFEL is of benefit for de novo phasing in the use of routinely used heavy atoms and high resolution data collection. |*Crystallography, X-Ray [MESH] |*Models, Molecular [MESH]An isomorphous replacement method for efficient de novo phasing for se(epmc).pdf DeepDyve Pubget Overpricing