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suck abstract from ncbi


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pmid25974523      Phys+Rev+E+Stat+Nonlin+Soft+Matter+Phys 2015 ; 91 (4): 042703
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  • Super-resolution two-photon microscopy via scanning patterned illumination #MMPMID25974523
  • Urban BE; Yi J; Chen S; Dong B; Zhu Y; DeVries SH; Backman V; Zhang HF
  • Phys Rev E Stat Nonlin Soft Matter Phys 2015[Apr]; 91 (4): 042703 PMID25974523show ga
  • We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microscopy. Using 2P-SPIM we achieved a lateral resolution of 141 nm, which represents an improvement by a factor of 1.9 over the corresponding diffraction limit. We further demonstrated super-resolution cellular imaging by 2P-SPIM to image actin cytoskeleton in mammalian cells and three-dimensional imaging in highly scattering retinal tissue.
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