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10.1038/srep13878 http://scihub22266oqcxt.onion/10.1038/srep13878 C4564737!4564737 !26354037     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26354037 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2015 ; 5 (ä): 13878 Nephropedia Template TP {{tp|p=26354037 |t=2015. Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system |pdf=|usr=}}{{26354037 }} gab.com Text Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26354037 #FOAvip Recent advances in the study of the CRISPR/Cas9 system have provided a precise and versatile approach for genome editing in various species. However, the applicability and efficiency of this method in large animal models, such as the goat, have not been extensively studied. Here, by co-injection of one-cell stage embryos with Cas9?mRNA and sgRNAs targeting two functional genes (MSTN and FGF5), we successfully produced gene-modified goats with either one or both genes disrupted. The targeting efficiency of MSTN and FGF5 in cultured primary fibroblasts was as high as 60%, while the efficiency of disrupting MSTN and FGF5 in 98 tested animals was 15% and 21% respectively, and 10% for double gene modifications. The on- and off-target mutations of the target genes in fibroblasts, as well as in somatic tissues and testis of founder and dead animals, were carefully analyzed. The results showed that simultaneous editing of several sites was achieved in large animals, demonstrating that the CRISPR/Cas9 system has the potential to become a robust and efficient gene engineering tool in farm animals, and therefore will be critically important and applicable for breeding. Twit Text FOAVip Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26354037 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26354037 #FOAvip English Wikipedia <ref>{{Cite pmid|26354037 }}</ref> Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system #MMPMID26354037 Wang X ; Yu H ; Lei A ; Zhou J ; Zeng W ; Zhu H ; Dong Z ; Niu Y ; Shi B ; Cai B ; Liu J ; Huang S ; Yan H ; Zhao X ; Zhou G ; He X ; Chen X ; Yang Y ; Jiang Y ; Shi L ; Tian X ; Wang Y ; Ma B ; Huang X ; Qu L ; Chen Y Sci Rep 2015[Sep]; 5 (ä): 13878 PMID26354037 show ga Recent advances in the study of the CRISPR/Cas9 system have provided a precise and versatile approach for genome editing in various species. However, the applicability and efficiency of this method in large animal models, such as the goat, have not been extensively studied. Here, by co-injection of one-cell stage embryos with Cas9?mRNA and sgRNAs targeting two functional genes (MSTN and FGF5), we successfully produced gene-modified goats with either one or both genes disrupted. The targeting efficiency of MSTN and FGF5 in cultured primary fibroblasts was as high as 60%, while the efficiency of disrupting MSTN and FGF5 in 98 tested animals was 15% and 21% respectively, and 10% for double gene modifications. The on- and off-target mutations of the target genes in fibroblasts, as well as in somatic tissues and testis of founder and dead animals, were carefully analyzed. The results showed that simultaneous editing of several sites was achieved in large animals, demonstrating that the CRISPR/Cas9 system has the potential to become a robust and efficient gene engineering tool in farm animals, and therefore will be critically important and applicable for breeding. |*Gene Targeting [MESH] |Animals [MESH] |Animals, Genetically Modified [MESH] |Base Sequence [MESH] |CRISPR-Cas Systems/*genetics [MESH] |Fibroblast Growth Factor 5/chemistry/*genetics [MESH] |Fibroblasts/metabolism [MESH] |Goats/*genetics [MESH] |Microinjections [MESH] |Molecular Sequence Data [MESH] |Myostatin/chemistry/*genetics [MESH] |Phenotype [MESH] |RNA Editing [MESH] |RNA, Guide, CRISPR-Cas Systems [MESH] |Sequence Alignment [MESH]Generation of gene-modified goats targeting MSTN and FGF5 via zygote i(epmc).pdf DeepDyve Pubget Overpricing