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10.1371/journal.pone.0137582 http://scihub22266oqcxt.onion/10.1371/journal.pone.0137582 C4564188!4564188 !26351848     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26351848 &cmd=llinks): Failed to open stream: HTTP request failed! 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UPR Activation and the Down-Regulation of ?-Crystallin in Human High Myopia-Related Cataract Lens Epithelium |pdf=|usr=}}{{26351848 }} gab.com Text UPR Activation and the Down-Regulation of -Crystallin in Human High Myopia-Related Cataract Lens Epithelium JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26351848 #FOAvip PURPOSE: To investigate the expression of ?A- and ?B-crystallin and the unfolded protein response in the lens epithelium of patients with high myopia-related cataracts. METHODS AND MATERIALS: The central portion of the human anterior lens capsule together with the adhering epithelial cells, approximately 5 mm in diameter, were harvested and processed within two hours after cataract surgery from high myopia-related (spherical equivalent ?-10.00 diopters) and age-related cataract patients or from high myopia but non-cataractous patients (tissue were collected from ocular trauma patients with high myopia and lens trauma). Anterior lens samples from fresh cadaver normal human eyes were used as normal control (collected within 6 hours from death). Real-time PCR was performed to detect the mRNA levels of ?-crystallins as well as unfolded protein response (UPR)-related GRP78, spliced-XBP1, ATF4 and ATF6. Western blot analysis was used to determine the protein level of ?-crystallin, GRP78, p-IRE1?, p-eIF2? and ATF6. RESULTS: In the lens epithelium of the high myopia-related cataract group and the age related cataract group, the mRNA and soluble protein expression of ?A- and ?B-crystallin were both decreased; additionally, the protein levels of ATF6, p-eIF2? and p-IRE1? and the gene expression levels of spliced XBP1, GRP78, ATF6 and ATF4 were greatly increased relative to the normal control. CONCLUSION: These results suggest the significant loss of soluble ?-crystallin and the activation of the UPR in the lens epithelium of patients with high myopia-related cataract, which may be associated with the cataractogenesis of high myopia-related cataract. Twit Text FOAVip UPR Activation and the Down-Regulation of -Crystallin in Human High Myopia-Related Cataract Lens Epithelium ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26351848 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26351848 #FOAvip English Wikipedia <ref>{{Cite pmid|26351848 }}</ref> UPR Activation and the Down-Regulation of ?-Crystallin in Human High Myopia-Related Cataract Lens Epithelium #MMPMID26351848 Yang J ; Zhou S ; Gu J ; Guo M ; Xia H ; Liu Y PLoS One 2015[]; 10 (9 ): e0137582 PMID26351848 show ga PURPOSE: To investigate the expression of ?A- and ?B-crystallin and the unfolded protein response in the lens epithelium of patients with high myopia-related cataracts. METHODS AND MATERIALS: The central portion of the human anterior lens capsule together with the adhering epithelial cells, approximately 5 mm in diameter, were harvested and processed within two hours after cataract surgery from high myopia-related (spherical equivalent ?-10.00 diopters) and age-related cataract patients or from high myopia but non-cataractous patients (tissue were collected from ocular trauma patients with high myopia and lens trauma). Anterior lens samples from fresh cadaver normal human eyes were used as normal control (collected within 6 hours from death). Real-time PCR was performed to detect the mRNA levels of ?-crystallins as well as unfolded protein response (UPR)-related GRP78, spliced-XBP1, ATF4 and ATF6. Western blot analysis was used to determine the protein level of ?-crystallin, GRP78, p-IRE1?, p-eIF2? and ATF6. RESULTS: In the lens epithelium of the high myopia-related cataract group and the age related cataract group, the mRNA and soluble protein expression of ?A- and ?B-crystallin were both decreased; additionally, the protein levels of ATF6, p-eIF2? and p-IRE1? and the gene expression levels of spliced XBP1, GRP78, ATF6 and ATF4 were greatly increased relative to the normal control. CONCLUSION: These results suggest the significant loss of soluble ?-crystallin and the activation of the UPR in the lens epithelium of patients with high myopia-related cataract, which may be associated with the cataractogenesis of high myopia-related cataract. |Adult [MESH] |Cataract/complications/*genetics/pathology [MESH] |Endoplasmic Reticulum Chaperone BiP [MESH] |Endoplasmic Reticulum/metabolism [MESH] |Epithelium/metabolism/pathology [MESH] |Female [MESH] |Gene Expression Regulation [MESH] |Humans [MESH] |Male [MESH] |Middle Aged [MESH] |Myopia/complications/*genetics/pathology [MESH] |Proteomics [MESH] |Unfolded Protein Response/genetics [MESH] |alpha-Crystallin A Chain/biosynthesis/*genetics [MESH]UPR Activation and the Down-Regulation of ?-Crystallin in Human High (epmc).pdf DeepDyve Pubget Overpricing