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10.3892/ijmm.2015.2318 http://scihub22266oqcxt.onion/10.3892/ijmm.2015.2318 C4564074!4564074 !26311239     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26311239 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Int+J+Mol+Med 2015 ; 36 (4 ): 967-74 Nephropedia Template TP {{tp|p=26311239 |t=2015. 1?,25-Dihydroxyvitamin D3 prevents the differentiation of human lung fibroblasts via microRNA-27b targeting the vitamin D receptor |pdf=|usr=}}{{26311239 }} gab.com Text 1 ,25-Dihydroxyvitamin D3 prevents the differentiation of human lung fibroblasts via microRNA-27b targeting the vitamin D receptor JO: Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=26311239 #FOAvip Pulmonary fibroblasts have key roles in the formation and maintenance of lung structure and function, and are involved in tissue repair and remodeling. Transforming growth factor??1 (TGF??1) induces differentiation of fibroblasts into myofibroblasts, the key effector cells in fibrotic states, which are characterized by the expression of ??smooth muscle actin (??SMA) markers. 1?,25?Dihydroxyvitamin D3 [1,25(OH)2D3] has been implicated in regulating differentiation, and the vitamin D receptor (VDR) may be a regulator of TGF?? signaling. In addition, there is presently only limited information regarding microRNA (miRNA) regulation of lung fibroblast differentiation. To determine the role of 1,25(OH)2D3 in regulating the differentiation of fibroblasts induced by TGF??1 and the functional importance of miR?27b, cell culture systems, cell transfection and the 3' untranslated region (3'UTR) luciferase assay were employed. 1,25(OH)2D3 inhibited differentiation and downregulated miR?27b expression in human lung fibroblasts induced by TGF??1. In addition, human lung fibroblasts were transfected with miR?27b mimic or miR?27b inhibitor, and demonstrated that the overexpression of miR?27b decreased the VDR protein expression and increased the expression of ??SMA, while reducing levels of miR?27b had opposing effects. Finally, the luciferase reporter assays were performed to confirm that miR?27b directly targeted VDR 3'UTR. Taken together, these results suggest that 1,25(OH)2D3 inhibits lung fibroblast differentiation induced by TGF??1 via miR?27b targeting VDR 3'UTR, which may be used as a novel treatment strategy in differentiation pathways. Twit Text FOAVip 1 ,25-Dihydroxyvitamin D3 prevents the differentiation of human lung fibroblasts via microRNA-27b targeting the vitamin D receptor ????Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=26311239 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26311239 #FOAvip English Wikipedia <ref>{{Cite pmid|26311239 }}</ref> 1?,25-Dihydroxyvitamin D3 prevents the differentiation of human lung fibroblasts via microRNA-27b targeting the vitamin D receptor #MMPMID26311239 Li F ; Zhang A ; Shi Y ; Ma Y ; Du Y Int J Mol Med 2015[Oct]; 36 (4 ): 967-74 PMID26311239 show ga Pulmonary fibroblasts have key roles in the formation and maintenance of lung structure and function, and are involved in tissue repair and remodeling. Transforming growth factor??1 (TGF??1) induces differentiation of fibroblasts into myofibroblasts, the key effector cells in fibrotic states, which are characterized by the expression of ??smooth muscle actin (??SMA) markers. 1?,25?Dihydroxyvitamin D3 [1,25(OH)2D3] has been implicated in regulating differentiation, and the vitamin D receptor (VDR) may be a regulator of TGF?? signaling. In addition, there is presently only limited information regarding microRNA (miRNA) regulation of lung fibroblast differentiation. To determine the role of 1,25(OH)2D3 in regulating the differentiation of fibroblasts induced by TGF??1 and the functional importance of miR?27b, cell culture systems, cell transfection and the 3' untranslated region (3'UTR) luciferase assay were employed. 1,25(OH)2D3 inhibited differentiation and downregulated miR?27b expression in human lung fibroblasts induced by TGF??1. In addition, human lung fibroblasts were transfected with miR?27b mimic or miR?27b inhibitor, and demonstrated that the overexpression of miR?27b decreased the VDR protein expression and increased the expression of ??SMA, while reducing levels of miR?27b had opposing effects. Finally, the luciferase reporter assays were performed to confirm that miR?27b directly targeted VDR 3'UTR. Taken together, these results suggest that 1,25(OH)2D3 inhibits lung fibroblast differentiation induced by TGF??1 via miR?27b targeting VDR 3'UTR, which may be used as a novel treatment strategy in differentiation pathways. |3' Untranslated Regions/physiology [MESH] |Actins/biosynthesis/genetics [MESH] |Calcitriol/*pharmacology [MESH] |Cell Differentiation/*drug effects/genetics [MESH] |Fibroblasts/cytology/*metabolism [MESH] |Gene Expression Regulation/drug effects/genetics [MESH] |Humans [MESH] |Lung/cytology/*metabolism [MESH] |MicroRNAs/genetics/*metabolism [MESH] |Receptors, Calcitriol/agonists/genetics/*metabolism [MESH] |Signal Transduction/*drug effects/genetics [MESH]1?,25-Dihydroxyvitamin D3 prevents the differentiation of human lung f(epmc).pdf DeepDyve Pubget Overpricing