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2015 ; 6
(ä): 940
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Nonlinear electrical impedance spectroscopy of viruses using very high electric
fields created by nanogap electrodes
#MMPMID26441875
Hatsuki R
; Honda A
; Kajitani M
; Yamamoto T
Front Microbiol
2015[]; 6
(ä): 940
PMID26441875
show ga
Our living sphere is constantly exposed to a wide range of pathogenic viruses,
which can be either known, or of novel origin. Currently, there is no methodology
for continuously monitoring the environment for viruses in general, much less a
methodology that allows the rapid and sensitive identification of a wide variety
of viruses responsible for communicable diseases. Traditional approaches, based
on PCR and immunodetection systems, only detect known or specifically targeted
viruses. We here describe a simple device that can potentially detect any virus
between nanogap electrodes using nonlinear impedance spectroscopy. Three test
viruses, differing in shape and size, were used to demonstrate the general
applicability of this approach: baculovirus, tobacco mosaic virus (TMV), and
influenza virus. We show that each of the virus types responded differently in
the nanogap to changes in the electric field strength, and the impedance of the
virus solutions differed depending both on virus type and virus concentration.
These preliminary results show that the three virus types can be distinguished
and their approximate concentrations determined. Although further studies are
required, the proposed nonlinear impedance spectroscopy method may achieve a
sensitivity comparable to that of more traditional, but less versatile, virus
detection systems.