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10.1038/srep13818 http://scihub22266oqcxt.onion/10.1038/srep13818 C4562301!4562301!26348662     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2015 ; 5 (ä): ä Nephropedia Template TP {{tp|p=26348662|t=2015. Electroporation of mammalian cells by nanosecond electric field oscillations and its inhibition by the electric field reversal |pdf=|usr=}}{{26348662}} gab.com Text Electroporation of mammalian cells by nanosecond electric field oscillations and its inhibition by the electric field reversal JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26348662 #FOAvip The present study compared electroporation efficiency of bipolar and unipolar nanosecond electric field oscillations (NEFO). Bipolar NEFO was a damped sine wave with 140?ns first phase duration at 50% height; the peak amplitude of phases 2?4 decreased to 35%, 12%, and 7% of the first phase. This waveform was rectified to produce unipolar NEFO by cutting off phases 2 and 4. Membrane permeabilization was quantified in CHO and GH3 cells by uptake of a membrane integrity marker dye YO-PRO-1 (YP) and by the membrane conductance increase measured by patch clamp. For treatments with 1?20 unipolar NEFO, at 9.6?24?kV/cm, 10?Hz, the rate and amount of YP uptake were consistently 2-3-fold higher than after bipolar NEFO treatments, despite delivering less energy. However, the threshold amplitude was about 7?kV/cm for both NEFO waveforms. A single 14.4?kV/cm unipolar NEFO caused a 1.5?2 times greater increase in membrane conductance (p?<?0.05) than bipolar NEFO, along with a longer and less frequent recovery. The lower efficiency of bipolar NEFO was preserved in Ca2+-free conditions and thus cannot be explained by the reversal of electrophoretic flows of Ca2+. Instead, the data indicate that the electric field polarity reversals reduced the pore yield. Twit Text FOAVip Electroporation of mammalian cells by nanosecond electric field oscillations and its inhibition by the electric field reversal ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26348662 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26348662 #FOAvip English Wikipedia <ref>{{Cite pmid|26348662}}</ref> Electroporation of mammalian cells by nanosecond electric field oscillations and its inhibition by the electric field reversal #MMPMID26348662 Gianulis EC; Lee J; Jiang C; Xiao S; Ibey BL; Pakhomov AG Sci Rep 2015[]; 5 (ä): ä PMID26348662show ga The present study compared electroporation efficiency of bipolar and unipolar nanosecond electric field oscillations (NEFO). Bipolar NEFO was a damped sine wave with 140?ns first phase duration at 50% height; the peak amplitude of phases 2?4 decreased to 35%, 12%, and 7% of the first phase. This waveform was rectified to produce unipolar NEFO by cutting off phases 2 and 4. Membrane permeabilization was quantified in CHO and GH3 cells by uptake of a membrane integrity marker dye YO-PRO-1 (YP) and by the membrane conductance increase measured by patch clamp. For treatments with 1?20 unipolar NEFO, at 9.6?24?kV/cm, 10?Hz, the rate and amount of YP uptake were consistently 2-3-fold higher than after bipolar NEFO treatments, despite delivering less energy. However, the threshold amplitude was about 7?kV/cm for both NEFO waveforms. A single 14.4?kV/cm unipolar NEFO caused a 1.5?2 times greater increase in membrane conductance (p? äElectroporation of mammalian cells by nanosecond electric field oscill(epmc).pdf DeepDyve Pubget Overpricing