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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Front+Bioeng+Biotechnol
2015 ; 3
(ä): 133
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English Wikipedia
High-Resolution X-Ray Techniques as New Tool to Investigate the 3D
Vascularization of Engineered-Bone Tissue
#MMPMID26442248
Bukreeva I
; Fratini M
; Campi G
; Pelliccia D
; Spanò R
; Tromba G
; Brun F
; Burghammer M
; Grilli M
; Cancedda R
; Cedola A
; Mastrogiacomo M
Front Bioeng Biotechnol
2015[]; 3
(ä): 133
PMID26442248
show ga
The understanding of structure-function relationships in normal and pathologic
mammalian tissues is at the basis of a tissue engineering (TE) approach for the
development of biological substitutes to restore or improve tissue function. In
this framework, it is interesting to investigate engineered bone tissue, formed
when porous ceramic constructs are loaded with bone marrow stromal cells (BMSC)
and implanted in vivo. To monitor the relation between bone formation and
vascularization, it is important to achieve a detailed imaging and a quantitative
description of the complete three-dimensional vascular network in such
constructs. Here, we used synchrotron X-ray phase-contrast micro-tomography to
visualize and analyze the three-dimensional micro-vascular networks in
bone-engineered constructs, in an ectopic bone formation mouse-model. We compared
samples seeded and not seeded with BMSC, as well as samples differently stained
or unstained. Thanks to the high quality of the images, we investigated the 3D
distribution of both vessels and collagen matrix and we obtained quantitative
information for all different samples. We propose our approach as a tool for
quantitative studies of angiogenesis in TE and for any pre-clinical investigation
where a quantitative analysis of the vascular network is required.