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10.1016/j.molcel.2015.06.037 http://scihub22266oqcxt.onion/10.1016/j.molcel.2015.06.037 C4561013!4561013 !26257284     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26257284 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Cell 2015 ; 59 (5 ): 768-80 Nephropedia Template TP {{tp|p=26257284 |t=2015. Crosslink Mapping at Amino Acid-Base Resolution Reveals the Path of Scrunched DNA in Initial Transcribing Complexes |pdf=|usr=}}{{26257284 }} gab.com Text Crosslink Mapping at Amino Acid-Base Resolution Reveals the Path of Scrunched DNA in Initial Transcribing Complexes JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=26257284 #FOAvip RNA polymerase binds tightly to DNA to recognize promoters with high specificity but then releases these contacts during the initial stage of transcription. We report a site-specific crosslinking approach to map the DNA path in bacterial transcription intermediates at amino acid and nucleotide resolution. After validating the approach by showing that the DNA path in open complexes (RPO) is the same as in high-resolution X-ray structures, we define the path following substrate addition in "scrunched" complexes (RPITC). The DNA bulges that form within the transcription bubble in RPITC are positioned differently on the two strands. Our data suggest that the non-template strand bulge is extruded into solvent in complexes containing a 5-mer RNA, whereas the template strand bulge remains within the template strand tunnel, exerting stress on interactions between the ? flap, ?' clamp, and ?3.2. We propose that this stress contributes to ?3.2 displacement from the RNA exit channel, facilitating promoter escape. Twit Text FOAVip Crosslink Mapping at Amino Acid-Base Resolution Reveals the Path of Scrunched DNA in Initial Transcribing Complexes ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=26257284 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26257284 #FOAvip English Wikipedia <ref>{{Cite pmid|26257284 }}</ref> Crosslink Mapping at Amino Acid-Base Resolution Reveals the Path of Scrunched DNA in Initial Transcribing Complexes #MMPMID26257284 Winkelman JT ; Winkelman BT ; Boyce J ; Maloney MF ; Chen AY ; Ross W ; Gourse RL Mol Cell 2015[Sep]; 59 (5 ): 768-80 PMID26257284 show ga RNA polymerase binds tightly to DNA to recognize promoters with high specificity but then releases these contacts during the initial stage of transcription. We report a site-specific crosslinking approach to map the DNA path in bacterial transcription intermediates at amino acid and nucleotide resolution. After validating the approach by showing that the DNA path in open complexes (RPO) is the same as in high-resolution X-ray structures, we define the path following substrate addition in "scrunched" complexes (RPITC). The DNA bulges that form within the transcription bubble in RPITC are positioned differently on the two strands. Our data suggest that the non-template strand bulge is extruded into solvent in complexes containing a 5-mer RNA, whereas the template strand bulge remains within the template strand tunnel, exerting stress on interactions between the ? flap, ?' clamp, and ?3.2. We propose that this stress contributes to ?3.2 displacement from the RNA exit channel, facilitating promoter escape. |Amino Acids/chemistry [MESH] |Base Sequence [MESH] |Cross-Linking Reagents [MESH] |Crystallography, X-Ray [MESH] |DNA, Bacterial/*chemistry/genetics/*metabolism [MESH] |DNA-Directed RNA Polymerases/chemistry/genetics/metabolism [MESH] |Escherichia coli Proteins/chemistry/genetics/metabolism [MESH] |Escherichia coli/genetics/metabolism [MESH] |Models, Molecular [MESH] |Nucleic Acid Conformation [MESH] |Promoter Regions, Genetic [MESH] |Protein Conformation [MESH] |Transcription, Genetic [MESH]Crosslink Mapping at Amino Acid-Base Resolution Reveals the Path of Sc(epmc).pdf DeepDyve Pubget Overpricing