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10.1016/j.molcel.2015.07.023 http://scihub22266oqcxt.onion/10.1016/j.molcel.2015.07.023 C4560836!4560836!26340425     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell 2015 ; 59 (5): 858-66 Nephropedia Template TP {{tp|p=26340425|t=2015. Tracking distinct RNA populations using efficient and reversible covalent chemistry |pdf=|usr=}}{{26340425}} gab.com Text Tracking distinct RNA populations using efficient and reversible covalent chemistry JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=26340425 #FOAvip We describe a chemical method to label and purify 4-thiouridine (s4U)-containing RNA. We demonstrate that methanethiosulfonate (MTS) reagents form disulfide bonds with s4U more efficiently than the commonly used HPDP-biotin, leading to higher yields and less biased enrichment. This increase in efficiency allowed us to use s4U-labeling to study global microRNA (miRNA) turnover in proliferating cultured human cells without perturbing global miRNA levels or the miRNA processing machinery. This improved chemistry will enhance methods that depend on tracking different populations of RNA, such as 4-thiouridine-tagging to study tissue-specific transcription and dynamic transcriptome analysis (DTA) to study RNA turnover. Twit Text FOAVip Tracking distinct RNA populations using efficient and reversible covalent chemistry ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=26340425 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26340425 #FOAvip English Wikipedia <ref>{{Cite pmid|26340425}}</ref> Tracking distinct RNA populations using efficient and reversible covalent chemistry #MMPMID26340425 Duffy EE; Rutenberg-Schoenberg M; Stark CD; Kitchen RR; Gerstein MB; Simon MD Mol Cell 2015[Sep]; 59 (5): 858-66 PMID26340425show ga We describe a chemical method to label and purify 4-thiouridine (s4U)-containing RNA. We demonstrate that methanethiosulfonate (MTS) reagents form disulfide bonds with s4U more efficiently than the commonly used HPDP-biotin, leading to higher yields and less biased enrichment. This increase in efficiency allowed us to use s4U-labeling to study global microRNA (miRNA) turnover in proliferating cultured human cells without perturbing global miRNA levels or the miRNA processing machinery. This improved chemistry will enhance methods that depend on tracking different populations of RNA, such as 4-thiouridine-tagging to study tissue-specific transcription and dynamic transcriptome analysis (DTA) to study RNA turnover. äTracking distinct RNA populations using efficient and reversible coval(epmc).pdf DeepDyve Pubget Overpricing