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10.1371/journal.pone.0136712 http://scihub22266oqcxt.onion/10.1371/journal.pone.0136712 C4560384!4560384!26340021     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2015 ; 10 (9): ä Nephropedia Template TP {{tp|p=26340021|t=2015. AFAP1 Is a Novel Downstream Mediator of TGF-?1 for CCN2 Induction in Osteoblasts |pdf=|usr=}}{{26340021}} gab.com Text AFAP1 Is a Novel Downstream Mediator of TGF- 1 for CCN2 Induction in Osteoblasts JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26340021 #FOAvip Background: CCN2 acts as an anabolic growth factor to regulate osteoblast differentiation and function. CCN2 is induced by TGF-?1 and acts as a mediator of TGF-?1 induced matrix production in osteoblasts and Src is required for CCN2 induction by TGF-?1; however, the molecular mechanisms that control CCN2 induction in osteoblasts are poorly understood. AFAP1 binds activated forms of Src and can direct the activation of Src in certain cell types, however a role for AFAP1 downstream of TGF-?1 or in osteoblats is undefined. In this study, we investigated the role of AFAP1 for CCN2 induction by TGF-?1 in primary osteoblasts. Results: We demonstrated that AFAP1 expression in osteoblasts occurs in a biphasic pattern with maximal expression levels occurring during osteoblast proliferation (~day 3), reduced expression during matrix production/maturation (~day 14?21), an a further increase in expression during mineralization (~day 21). AFAP1 expression is induced by TGF-?1 treatment in osteoblasts during days 7, 14 and 21. In osteoblasts, AFAP1 binds to Src and is required for Src activation by TGF-?1 and CCN2 promoter activity and protein induction by TGF-?1 treatment was impaired using AFAP1 siRNA, indicating the requirement of AFAP1 for CCN2 induction by TGF-?1. We also demonstrated that TGF-?1 induction of extracellular matrix protein collagen XIIa occurs in an AFAP1 dependent fashion. Conclusions: This study demonstrates that AFAP1 is an essential downstream signaling component of TGF-?1 for Src activation, CCN2 induction and collagen XIIa in osteoblasts. Twit Text FOAVip AFAP1 Is a Novel Downstream Mediator of TGF- 1 for CCN2 Induction in Osteoblasts ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26340021 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26340021 #FOAvip English Wikipedia <ref>{{Cite pmid|26340021}}</ref> AFAP1 Is a Novel Downstream Mediator of TGF-?1 for CCN2 Induction in Osteoblasts #MMPMID26340021 Cho Y; Silverstein R; Geisinger MT; Martinkovich S; Corkill H; Cunnick JM; Planey SL; Arnott JA PLoS One 2015[]; 10 (9): ä PMID26340021show ga Background: CCN2 acts as an anabolic growth factor to regulate osteoblast differentiation and function. CCN2 is induced by TGF-?1 and acts as a mediator of TGF-?1 induced matrix production in osteoblasts and Src is required for CCN2 induction by TGF-?1; however, the molecular mechanisms that control CCN2 induction in osteoblasts are poorly understood. AFAP1 binds activated forms of Src and can direct the activation of Src in certain cell types, however a role for AFAP1 downstream of TGF-?1 or in osteoblats is undefined. In this study, we investigated the role of AFAP1 for CCN2 induction by TGF-?1 in primary osteoblasts. Results: We demonstrated that AFAP1 expression in osteoblasts occurs in a biphasic pattern with maximal expression levels occurring during osteoblast proliferation (~day 3), reduced expression during matrix production/maturation (~day 14?21), an a further increase in expression during mineralization (~day 21). AFAP1 expression is induced by TGF-?1 treatment in osteoblasts during days 7, 14 and 21. In osteoblasts, AFAP1 binds to Src and is required for Src activation by TGF-?1 and CCN2 promoter activity and protein induction by TGF-?1 treatment was impaired using AFAP1 siRNA, indicating the requirement of AFAP1 for CCN2 induction by TGF-?1. We also demonstrated that TGF-?1 induction of extracellular matrix protein collagen XIIa occurs in an AFAP1 dependent fashion. Conclusions: This study demonstrates that AFAP1 is an essential downstream signaling component of TGF-?1 for Src activation, CCN2 induction and collagen XIIa in osteoblasts. äAFAP1 Is a Novel Downstream Mediator of TGF-?1 for CCN2 Induction in O(epmc).pdf DeepDyve Pubget Overpricing