Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1002/art.38754 http://scihub22266oqcxt.onion/10.1002/art.38754 C4560105!4560105!24965753     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Arthritis+Rheumatol 2014 ; 66 (10): 2706-15 Nephropedia Template TP {{tp|p=24965753|t=2014. Barcode-Enabled Sequencing of Plasmablast Antibody Repertoires in Rheumatoid Arthritis |pdf=|usr=}}{{24965753}} gab.com Text Barcode-Enabled Sequencing of Plasmablast Antibody Repertoires in Rheumatoid Arthritis JO: Arthritis Rheumatol http://www.kidney.de/pget.php?&tw=1&pmid=24965753 #FOAvip Objective: A hallmark of rheumatoid arthritis (RA) is the production of autoantibodies, including anti-citrullinated protein antibodies (ACPAs). Nevertheless, the specific targets of these autoantibodies remain incompletely defined. During an immune response, B cells specific for the inciting antigen(s) are activated and differentiate into ?plasmablasts?, which are released into the blood. In this study we sequence the plasmablast antibody repertoire to define the targets of the active immune response in RA. Methods: We developed a novel DNA barcoding method to sequence the cognate heavy- and light-chain pairs of antibodies expressed by individual blood plasmablasts in RA. The method uses a universal 5? adapter that enables full-length sequencing of the antibodies? variable regions and recombinant expression of the paired antibody chains. The sequence datasets were bioinformatically analyzed to generate phylogenetic trees that identify clonal families of antibodies sharing heavy- and light-chain VJ sequences. Representative antibodies were expressed, and their binding properties characterized using CCP2 ELISA and antigen microarrays. Results: We used our sequencing method to generate phylogenetic trees representing the antibody repertoires of peripheral blood plasmablasts of 4 individuals with anti-CCP+ RA, and recombinantly expressed 14 antibodies that were either ?singleton? antibodies or representative of clonal antibody families. CCP2 ELISA identified four ACPAs, and antigen microarray analysis identified ACPAs that differentially targeted epitopes on ?-enolase, citrullinated fibrinogen, and citrullinated histone 2B. Conclusions: Our data provide evidence that autoantibodies targeting ?-enolase, citrullinated fibrinogen, and citrullinated histone 2B are produced by the ongoing activated B cell response in, and thus may contribute to the pathogenesis of, RA. Twit Text FOAVip Barcode-Enabled Sequencing of Plasmablast Antibody Repertoires in Rheumatoid Arthritis ????Arthritis Rheumatol http://www.kidney.de/pget.php?&tw=1&pmid=24965753 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24965753 #FOAvip English Wikipedia <ref>{{Cite pmid|24965753}}</ref> Barcode-Enabled Sequencing of Plasmablast Antibody Repertoires in Rheumatoid Arthritis #MMPMID24965753 Tan YC; Kongpachith S; Blum LK; Ju CH; Lahey LJ; Lu DR; Cai X; Wagner CA; Lindstrom TM; Sokolove J; Robinson WH Arthritis Rheumatol 2014[Oct]; 66 (10): 2706-15 PMID24965753show ga Objective: A hallmark of rheumatoid arthritis (RA) is the production of autoantibodies, including anti-citrullinated protein antibodies (ACPAs). Nevertheless, the specific targets of these autoantibodies remain incompletely defined. During an immune response, B cells specific for the inciting antigen(s) are activated and differentiate into ?plasmablasts?, which are released into the blood. In this study we sequence the plasmablast antibody repertoire to define the targets of the active immune response in RA. Methods: We developed a novel DNA barcoding method to sequence the cognate heavy- and light-chain pairs of antibodies expressed by individual blood plasmablasts in RA. The method uses a universal 5? adapter that enables full-length sequencing of the antibodies? variable regions and recombinant expression of the paired antibody chains. The sequence datasets were bioinformatically analyzed to generate phylogenetic trees that identify clonal families of antibodies sharing heavy- and light-chain VJ sequences. Representative antibodies were expressed, and their binding properties characterized using CCP2 ELISA and antigen microarrays. Results: We used our sequencing method to generate phylogenetic trees representing the antibody repertoires of peripheral blood plasmablasts of 4 individuals with anti-CCP+ RA, and recombinantly expressed 14 antibodies that were either ?singleton? antibodies or representative of clonal antibody families. CCP2 ELISA identified four ACPAs, and antigen microarray analysis identified ACPAs that differentially targeted epitopes on ?-enolase, citrullinated fibrinogen, and citrullinated histone 2B. Conclusions: Our data provide evidence that autoantibodies targeting ?-enolase, citrullinated fibrinogen, and citrullinated histone 2B are produced by the ongoing activated B cell response in, and thus may contribute to the pathogenesis of, RA. äBarcode-Enabled Sequencing of Plasmablast Antibody Repertoires in Rheu(epmc).pdf DeepDyve Pubget Overpricing