Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1038/srep13670 http://scihub22266oqcxt.onion/10.1038/srep13670 C4557066!4557066 !26329726     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26329726 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26329726 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2015 ; 5 (ä): 13670 Nephropedia Template TP {{tp|p=26329726 |t=2015. Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources |pdf=|usr=}}{{26329726 }} gab.com Text Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26329726 #FOAvip Plant secondary metabolites have been attracting people's attention for centuries, due to their potentials; however, their production is still difficult and costly. The rich diversity of microbes and microbial genome sequence data provide unprecedented gene resources that enable to develop efficient artificial pathways in microorganisms. Here, by mimicking a natural pathway of plants using microbial genes, a new metabolic route was developed in E. coli for the synthesis of vanillin, the most widely used flavoring agent. A series of factors were systematically investigated for raising production, including efficiency and suitability of genes, gene dosage, and culture media. The metabolically engineered strain produced 97.2?mg/L vanillin from l-tyrosine, 19.3?mg/L from glucose, 13.3?mg/L from xylose and 24.7?mg/L from glycerol. These results show that the metabolic route enables production of natural vanillin from low-cost substrates, suggesting that it is a good strategy to mimick natural pathways for artificial pathway design. Twit Text FOAVip Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26329726 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26329726 #FOAvip English Wikipedia <ref>{{Cite pmid|26329726 }}</ref> Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources #MMPMID26329726 Ni J ; Tao F ; Du H ; Xu P Sci Rep 2015[Sep]; 5 (ä): 13670 PMID26329726 show ga Plant secondary metabolites have been attracting people's attention for centuries, due to their potentials; however, their production is still difficult and costly. The rich diversity of microbes and microbial genome sequence data provide unprecedented gene resources that enable to develop efficient artificial pathways in microorganisms. Here, by mimicking a natural pathway of plants using microbial genes, a new metabolic route was developed in E. coli for the synthesis of vanillin, the most widely used flavoring agent. A series of factors were systematically investigated for raising production, including efficiency and suitability of genes, gene dosage, and culture media. The metabolically engineered strain produced 97.2?mg/L vanillin from l-tyrosine, 19.3?mg/L from glucose, 13.3?mg/L from xylose and 24.7?mg/L from glycerol. These results show that the metabolic route enables production of natural vanillin from low-cost substrates, suggesting that it is a good strategy to mimick natural pathways for artificial pathway design. |*Biosynthetic Pathways/drug effects/genetics [MESH] |Benzaldehydes/chemistry/*metabolism [MESH] |Carbon/*pharmacology [MESH] |Coumaric Acids/metabolism [MESH] |Escherichia coli/drug effects/genetics/metabolism [MESH] |Fermentation/drug effects [MESH] |Gene Expression Regulation, Bacterial/drug effects [MESH] |Mass Spectrometry [MESH] |Multigene Family [MESH] |Plasmids/metabolism [MESH] |Propanols/metabolism [MESH] |Recombination, Genetic/genetics [MESH]Mimicking a natural pathway for de novo biosynthesis: natural vanillin(epmc).pdf DeepDyve Pubget Overpricing