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2015 ; 2
(ä): 150046
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A resource from 3D electron microscopy of hippocampal neuropil for user training
and tool development
#MMPMID26347348
Harris KM
; Spacek J
; Bell ME
; Parker PH
; Lindsey LF
; Baden AD
; Vogelstein JT
; Burns R
Sci Data
2015[]; 2
(ä): 150046
PMID26347348
show ga
Resurgent interest in synaptic circuitry and plasticity has emphasized the
importance of 3D reconstruction from serial section electron microscopy (3DEM).
Three volumes of hippocampal CA1 neuropil from adult rat were imaged at X-Y
resolution of ~2?nm on serial sections of ~50-60?nm thickness. These are the
first densely reconstructed hippocampal volumes. All axons, dendrites, glia, and
synapses were reconstructed in a cube (~10??m(3)) surrounding a large dendritic
spine, a cylinder (~43??m(3)) surrounding an oblique dendritic segment (3.4??m
long), and a parallelepiped (~178??m(3)) surrounding an apical dendritic segment
(4.9??m long). The data provide standards for identifying ultrastructural objects
in 3DEM, realistic reconstructions for modeling biophysical properties of
synaptic transmission, and a test bed for enhancing reconstruction tools.
Representative synapses are quantified from varying section planes, and
microtubules, polyribosomes, smooth endoplasmic reticulum, and endosomes are
identified and reconstructed in a subset of dendrites. The original images,
traces, and Reconstruct software and files are freely available and visualized at
the Open Connectome Project (Data Citation 1).