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10.4196/kjpp.2015.19.5.441 http://scihub22266oqcxt.onion/10.4196/kjpp.2015.19.5.441 C4553404!4553404!26330757     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Korean+J+Physiol+Pharmacol 2015 ; 19 (5): 441-9 Nephropedia Template TP {{tp|p=26330757|t=2015. Scutellarein Reduces Inflammatory Responses by Inhibiting Src Kinase Activity |pdf=|usr=}}{{26330757}} gab.com Text Scutellarein Reduces Inflammatory Responses by Inhibiting Src Kinase Activity JO: Korean J Physiol Pharmacol http://www.kidney.de/pget.php?&tw=1&pmid=26330757 #FOAvip Flavonoids are plant pigments that have been demonstrated to exert various pharmacological effects including anti-cancer, anti-diabetic, anti-atherosclerotic, anti-bacterial, and anti-inflammatory activities. However, the molecular mechanisms in terms of exact target proteins of flavonoids are not fully elucidated yet. In this study, we aimed to evaluate the anti-inflammatory mechanism of scutellarein (SCT), a flavonoid isolated from Erigeron breviscapus, Clerodendrum phlomidis and Oroxylum indicum Vent that have been traditionally used to treat various inflammatory diseases in China and Brazil. For this purpose, a nitric oxide (NO) assay, polymerase chain reaction (PCR), nuclear fractionation, immunoblot analysis, a kinase assay, and an overexpression strategy were employed. Scutellarein significantly inhibited NO production in a dose-dependent manner and reduced the mRNA expression levels of inducible NO synthase (iNOS) and tumor necrosis factor (TNF)-? in lipopolysaccharide (LPS)-activated RAW264.7 cells. In addition, SCT also dampened nuclear factor (NF)-?B-driven expression of a luciferase reporter gene upon transfection of a TIR-domain-containing adapter-inducing interferon-? (TRIF) construct into Human embryonic kidney 293 (HEK 293) cells; similarly, NF-? B nuclear translocation was inhibited by SCT. Moreover, the phosphorylation levels of various upstream signaling enzymes involved in NF-?B activation were decreased by SCT treatment in LPS-treated RAW264.7 cells. Finally, SCT strongly inhibited Src kinase activity and also inhibited the autophosphorylation of overexpressed Src. Therefore, our data suggest that SCT can block the inflammatory response by directly inhibiting Src kinase activity linked to NF-?B activation. Twit Text FOAVip Scutellarein Reduces Inflammatory Responses by Inhibiting Src Kinase Activity ????Korean J Physiol Pharmacol http://www.kidney.de/pget.php?&tw=1&pmid=26330757 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26330757 #FOAvip English Wikipedia <ref>{{Cite pmid|26330757}}</ref> Scutellarein Reduces Inflammatory Responses by Inhibiting Src Kinase Activity #MMPMID26330757 Sung NY; Kim MY; Cho JY Korean J Physiol Pharmacol 2015[Sep]; 19 (5): 441-9 PMID26330757show ga Flavonoids are plant pigments that have been demonstrated to exert various pharmacological effects including anti-cancer, anti-diabetic, anti-atherosclerotic, anti-bacterial, and anti-inflammatory activities. However, the molecular mechanisms in terms of exact target proteins of flavonoids are not fully elucidated yet. In this study, we aimed to evaluate the anti-inflammatory mechanism of scutellarein (SCT), a flavonoid isolated from Erigeron breviscapus, Clerodendrum phlomidis and Oroxylum indicum Vent that have been traditionally used to treat various inflammatory diseases in China and Brazil. For this purpose, a nitric oxide (NO) assay, polymerase chain reaction (PCR), nuclear fractionation, immunoblot analysis, a kinase assay, and an overexpression strategy were employed. Scutellarein significantly inhibited NO production in a dose-dependent manner and reduced the mRNA expression levels of inducible NO synthase (iNOS) and tumor necrosis factor (TNF)-? in lipopolysaccharide (LPS)-activated RAW264.7 cells. In addition, SCT also dampened nuclear factor (NF)-?B-driven expression of a luciferase reporter gene upon transfection of a TIR-domain-containing adapter-inducing interferon-? (TRIF) construct into Human embryonic kidney 293 (HEK 293) cells; similarly, NF-? B nuclear translocation was inhibited by SCT. Moreover, the phosphorylation levels of various upstream signaling enzymes involved in NF-?B activation were decreased by SCT treatment in LPS-treated RAW264.7 cells. Finally, SCT strongly inhibited Src kinase activity and also inhibited the autophosphorylation of overexpressed Src. Therefore, our data suggest that SCT can block the inflammatory response by directly inhibiting Src kinase activity linked to NF-?B activation. äScutellarein Reduces Inflammatory Responses by Inhibiting Src Kinase A(epmc).pdf DeepDyve Pubget Overpricing