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10.1038/srep13591 http://scihub22266oqcxt.onion/10.1038/srep13591 C4551994!4551994!26314276     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2015 ; 5 (ä): ä Nephropedia Template TP {{tp|p=26314276|t=2015. Retinal synaptic regeneration via microfluidic guiding channels |pdf=|usr=}}{{26314276}} gab.com Text Retinal synaptic regeneration via microfluidic guiding channels JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26314276 #FOAvip In vitro culture of dissociated retinal neurons is an important model for investigating retinal synaptic regeneration (RSR) and exploring potentials in artificial retina. Here, retinal precursor cells were cultured in a microfluidic chip with multiple arrays of microchannels in order to reconstruct the retinal neuronal synapse. The cultured retinal cells were physically connected through microchannels. Activation of electric signal transduction by the cells through the microchannels was demonstrated by administration of glycinergic factors. In addition, an image-based analytical method was used to quantify the synaptic connections and to assess the kinetics of synaptic regeneration. The rate of RSR decreased significantly below 100??M of inhibitor glycine and then approached to a relatively constant level at higher concentrations. Furthermore, RSR was enhanced by chemical stimulation with potassium chloride. Collectively, the microfluidic synaptic regeneration chip provides a novel tool for high-throughput investigation of RSR at the cellular level and may be useful in quality control of retinal precursor cell transplantation. Twit Text FOAVip Retinal synaptic regeneration via microfluidic guiding channels ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26314276 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26314276 #FOAvip English Wikipedia <ref>{{Cite pmid|26314276}}</ref> Retinal synaptic regeneration via microfluidic guiding channels #MMPMID26314276 Su PJ; Liu Z; Zhang K; Han X; Saito Y; Xia X; Yokoi K; Shen H; Qin L Sci Rep 2015[]; 5 (ä): ä PMID26314276show ga In vitro culture of dissociated retinal neurons is an important model for investigating retinal synaptic regeneration (RSR) and exploring potentials in artificial retina. Here, retinal precursor cells were cultured in a microfluidic chip with multiple arrays of microchannels in order to reconstruct the retinal neuronal synapse. The cultured retinal cells were physically connected through microchannels. Activation of electric signal transduction by the cells through the microchannels was demonstrated by administration of glycinergic factors. In addition, an image-based analytical method was used to quantify the synaptic connections and to assess the kinetics of synaptic regeneration. The rate of RSR decreased significantly below 100??M of inhibitor glycine and then approached to a relatively constant level at higher concentrations. Furthermore, RSR was enhanced by chemical stimulation with potassium chloride. Collectively, the microfluidic synaptic regeneration chip provides a novel tool for high-throughput investigation of RSR at the cellular level and may be useful in quality control of retinal precursor cell transplantation. äRetinal synaptic regeneration via microfluidic guiding channels (epmc).pdf DeepDyve Pubget Overpricing