Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1128/AEM.00929-15 http://scihub22266oqcxt.onion/10.1128/AEM.00929-15 C4551229!4551229!26092450     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Appl+Environ+Microbiol 2015 ; 81 (17): 5794-803 Nephropedia Template TP {{tp|p=26092450|t=2015. Indirect Immunodetection of Fungal Fragments by Field Emission Scanning Electron Microscopy |pdf=|usr=}}{{26092450}} gab.com Text Indirect Immunodetection of Fungal Fragments by Field Emission Scanning Electron Microscopy JO: Appl Environ Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=26092450 #FOAvip Submicronic fungal fragments have been observed in in vitro aerosolization experiments. The occurrence of these particles has therefore been suggested to contribute to respiratory health problems observed in mold-contaminated indoor environments. However, the role of submicronic fragments in exacerbating adverse health effects has remained unclear due to limitations associated with detection methods. In the present study, we report the development of an indirect immunodetection assay that utilizes chicken polyclonal antibodies developed against spores from Aspergillus versicolor and high-resolution field emission scanning electron microscopy (FESEM). Immunolabeling was performed with A. versicolor fragments immobilized and fixed onto poly-l-lysine-coated polycarbonate filters. Ninety percent of submicronic fragments and 1- to 2-?m fragments, compared to 100% of >2-?m fragments generated from pure freeze-dried mycelial fragments of A. versicolor, were positively labeled. In proof-of-concept experiments, air samples collected from moldy indoor environments were evaluated using the immunolabeling technique. Our results indicated that 13% of the total collected particles were derived from fungi. This fraction comprises 79% of the fragments that were detected by immunolabeling and 21% of the spore particles that were morphologically identified. The methods reported in this study enable the enumeration of fungal particles, including submicronic fragments, in a complex heterogeneous environmental sample. Twit Text FOAVip Indirect Immunodetection of Fungal Fragments by Field Emission Scanning Electron Microscopy ????Appl Environ Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=26092450 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26092450 #FOAvip English Wikipedia <ref>{{Cite pmid|26092450}}</ref> Indirect Immunodetection of Fungal Fragments by Field Emission Scanning Electron Microscopy #MMPMID26092450 Afanou KA; Straumfors A; Skogstad A; Nayak AP; Skaar I; Hjeljord L; Tronsmo A; Eduard W; Green BJ Appl Environ Microbiol 2015[Sep]; 81 (17): 5794-803 PMID26092450show ga Submicronic fungal fragments have been observed in in vitro aerosolization experiments. The occurrence of these particles has therefore been suggested to contribute to respiratory health problems observed in mold-contaminated indoor environments. However, the role of submicronic fragments in exacerbating adverse health effects has remained unclear due to limitations associated with detection methods. In the present study, we report the development of an indirect immunodetection assay that utilizes chicken polyclonal antibodies developed against spores from Aspergillus versicolor and high-resolution field emission scanning electron microscopy (FESEM). Immunolabeling was performed with A. versicolor fragments immobilized and fixed onto poly-l-lysine-coated polycarbonate filters. Ninety percent of submicronic fragments and 1- to 2-?m fragments, compared to 100% of >2-?m fragments generated from pure freeze-dried mycelial fragments of A. versicolor, were positively labeled. In proof-of-concept experiments, air samples collected from moldy indoor environments were evaluated using the immunolabeling technique. Our results indicated that 13% of the total collected particles were derived from fungi. This fraction comprises 79% of the fragments that were detected by immunolabeling and 21% of the spore particles that were morphologically identified. The methods reported in this study enable the enumeration of fungal particles, including submicronic fragments, in a complex heterogeneous environmental sample. äIndirect Immunodetection of Fungal Fragments by Field Emission Scannin(epmc).pdf DeepDyve Pubget Overpricing