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10.1038/ncomms9083 http://scihub22266oqcxt.onion/10.1038/ncomms9083 C4544769!4544769!26278926     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2015 ; 6 (ä): ä Nephropedia Template TP {{tp|p=26278926|t=2015. Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries |pdf=|usr=}}{{26278926}} gab.com Text Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26278926 #FOAvip The CRISPR-Cas9 system is a powerful tool to edit eukaryotic genomes that has recently been adapted for functional screens. Several of its applications?including the disruption of genes using Cas9-nickase and the generation of large deletions?require co-expression of two distinct guide RNAs (gRNAs). However, the lack of experimental approaches to generate pools of paired gRNA vectors prevents these applications from being scalable. Here we report a simple, inexpensive, one-step method that allows for the rapid and efficient cloning of gRNA pairs into expression vectors. We show that this method can be used to generate pooled libraries and is therefore suitable for in vivo and in vitro functional screens. Twit Text FOAVip Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26278926 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26278926 #FOAvip English Wikipedia <ref>{{Cite pmid|26278926}}</ref> Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries #MMPMID26278926 Vidigal JA; Ventura A Nat Commun 2015[]; 6 (ä): ä PMID26278926show ga The CRISPR-Cas9 system is a powerful tool to edit eukaryotic genomes that has recently been adapted for functional screens. Several of its applications?including the disruption of genes using Cas9-nickase and the generation of large deletions?require co-expression of two distinct guide RNAs (gRNAs). However, the lack of experimental approaches to generate pools of paired gRNA vectors prevents these applications from being scalable. Here we report a simple, inexpensive, one-step method that allows for the rapid and efficient cloning of gRNA pairs into expression vectors. We show that this method can be used to generate pooled libraries and is therefore suitable for in vivo and in vitro functional screens. äRapid and efficient one-step generation of paired gRNA CRISPR-Cas9 lib(epmc).pdf DeepDyve Pubget Overpricing