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2015 ; 2015
(ä): 418752
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Comparative Analysis of the Hematopoietic Progenitor Cells from Placenta, Cord
Blood, and Fetal Liver, Based on Their Immunophenotype
#MMPMID26347038
Kuchma MD
; Kyryk VM
; Svitina HM
; Shablii YM
; Lukash LL
; Lobyntseva GS
; Shablii VA
Biomed Res Int
2015[]; 2015
(ä): 418752
PMID26347038
show ga
We have investigated the characteristics of human hematopoietic progenitor cells
(HPCs) with the CD34(+)CD45(low)SSC(low) phenotype from full-term placental
tissue (FTPT) as compared to cord blood (CB) and fetal liver (FL) cells. We
demonstrated the presence of cell subpopulations at various stages of the
differentiation with such immunophenotypes as CD34(+/low)CD45(low/-),
CD34(++)CD45(low/-), CD34(+++)CD45(low/-), CD34(+/low)CD45(hi), and
CD34(++)CD45(hi) in both first trimester placental tissue (FiTPT) and FTPT which
implies their higher phenotypic heterogeneity compared to CB. HPCs of the FTPT
origin expressed the CD90 antigen at a higher level compared to its expression by
the CB HPCs and the CD133 antigen expression being at the same level in both
cases. The HPCs compartment of FTPT versus CB contained higher number of myeloid
and erythroid committed cells but lower number of myeloid and lymphoid ones
compared to FL HPCs. HPCs of the FTPT and CB origin possess similar potentials
for the multilineage differentiation in vitro and similar ratios of myeloid and
erythroid progenitors among the committed cells. This observation suggests that
the active hematopoiesis occurs in the FTPT. We obtained viable HPCs from
cryopreserved placental tissue fragments allowing us to develop procedures for
banking and testing of placenta-derived HPCs for clinical use.