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2015 ; 10
(1
): 1-8
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Comparison of Prostatic Tissue Processed by Microwave and Conventional Technique
Using Morphometry
#MMPMID26516319
Jain P
; Kumar S
; Arora B
; Singh S
; Chabbra S
; Sen R
Iran J Pathol
2015[Win]; 10
(1
): 1-8
PMID26516319
show ga
BACKGROUND AND OBJECTIVES: Rapid processing of histopathological specimens and
decreased turnaround time is important to fulfill the needs of clinicians
treating sick patients, so the present study was conducted to compare the time
taken and quality of sections in processing of prostatic tissue by rapid
microwave and conventional techniques using morphometry. METHODS: Four to five mm
thick paired prostate tissue pieces of fifty cases of prostatectomy specimens
were taken. One tissue piece of the pair was processed routinely overnight by
conventional tissue processing and the other by microwave processing. Time taken
for processing by both conventional technique and microwave technique was noted
and compared. Then, both were stained with conventional method of hematoxylin and
eosin staining and examined for histological typing and grading. Morphometric
study was done on slides of prostatic tissue processed by both conventional and
microwave technique. RESULT: The prostatectomy specimens included both benign
(86%) and malignant (14%) prostatic lesions in the age range of 46-85 years. The
time taken for steps of dehydration, clearing and impregnation in microwave
technique was significantly less as compared to histoprocessing done by
conventional technique. Morphology, staining patterns of prostatic tissue
processed within minutes by microwave technique, whether benign or malignant,
were comparable to those sections which were processed in days using standard
technique. CONCLUSION: Domestic microwave oven can be used for histoprocessing to
accelerate the processing with preservation of morphology and is cheaper than
commercially available microwave ovens and processing time was considerably
reduced from days to minutes.