Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1038/srep12879 http://scihub22266oqcxt.onion/10.1038/srep12879 C4536488!4536488 !26271723     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26271723 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2015 ; 5 (ä): 12879 Nephropedia Template TP {{tp|p=26271723 |t=2015. Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer |pdf=|usr=}}{{26271723 }} gab.com Text Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26271723 #FOAvip Contact-dependent intercellular transfer (codeIT) of cellular constituents can have functional consequences for recipient cells, such as enhanced survival and drug resistance. Pathogenic viruses, prions and bacteria can also utilize this mechanism to spread to adjacent cells and potentially evade immune detection. However, little is known about the molecular mechanism underlying this intercellular transfer process. Here, we present a novel microscopy-based screening method to identify regulators and cargo of codeIT. Single donor cells, carrying fluorescently labelled endocytic organelles or proteins, are co-cultured with excess acceptor cells. CodeIT is quantified by confocal microscopy and image analysis in 3D, preserving spatial information. An siRNA-based screening using this method revealed the involvement of several myosins and small GTPases as codeIT regulators. Our data indicates that cellular protrusions and tubular recycling endosomes are important for codeIT. We automated image acquisition and analysis to facilitate large-scale chemical and genetic screening efforts to identify key regulators of codeIT. Twit Text FOAVip Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26271723 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26271723 #FOAvip English Wikipedia <ref>{{Cite pmid|26271723 }}</ref> Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer #MMPMID26271723 Frei DM ; Hodneland E ; Rios-Mondragon I ; Burtey A ; Neumann B ; Bulkescher J ; Schölermann J ; Pepperkok R ; Gerdes HH ; Kögel T Sci Rep 2015[Aug]; 5 (ä): 12879 PMID26271723 show ga Contact-dependent intercellular transfer (codeIT) of cellular constituents can have functional consequences for recipient cells, such as enhanced survival and drug resistance. Pathogenic viruses, prions and bacteria can also utilize this mechanism to spread to adjacent cells and potentially evade immune detection. However, little is known about the molecular mechanism underlying this intercellular transfer process. Here, we present a novel microscopy-based screening method to identify regulators and cargo of codeIT. Single donor cells, carrying fluorescently labelled endocytic organelles or proteins, are co-cultured with excess acceptor cells. CodeIT is quantified by confocal microscopy and image analysis in 3D, preserving spatial information. An siRNA-based screening using this method revealed the involvement of several myosins and small GTPases as codeIT regulators. Our data indicates that cellular protrusions and tubular recycling endosomes are important for codeIT. We automated image acquisition and analysis to facilitate large-scale chemical and genetic screening efforts to identify key regulators of codeIT. |Cell Communication/*physiology [MESH] |Cell Membrane/*physiology/ultrastructure [MESH] |Cell Tracking/methods [MESH] |GTP Phosphohydrolases/*metabolism [MESH] |HeLa Cells [MESH] |Humans [MESH] |Intercellular Junctions/*physiology [MESH] |Lab-On-A-Chip Devices [MESH] |Microscopy, Confocal/*methods [MESH] |Molecular Imaging/methods [MESH]Novel microscopy-based screening method reveals regulators of contact-(epmc).pdf DeepDyve Pubget Overpricing