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2014 ; 2
(ä): 189-91
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Gene expression profiling to define the cell intrinsic role of the SKI
proto-oncogene in hematopoiesis and myeloid neoplasms
#MMPMID26484093
Chalk AM
; Liddicoat BJ
; Walkley CR
; Singbrant S
Genom Data
2014[Dec]; 2
(ä): 189-91
PMID26484093
show ga
The proto-oncogene SKI is highly expressed in human myeloid leukemia and also in
murine hematopoietic stem cells. However, its operative relevance in these cells
remains elusive. We have over-expressed SKI to define its intrinsic role in
hematopoiesis and myeloid neoplasms, which resulted in a robust competitive
advantage upon transplantation, a complete dominance of the stem and progenitor
compartments, and a marked enhancement of myeloid differentiation at the expense
of other lineages. Accordingly, enforced expression of SKI induced gene
signatures associated with hematopoietic stem cells and myeloid differentiation.
Here we provide detailed experimental methods and analysis for the gene
expression profiling described in our recently published study of Singbrant et
al. (2014) in Haematologica. Our data sets (available at
http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39457) provide a resource
for exploring the underlying molecular mechanisms of the involvement of the
proto-oncogene SKI in hematopoietic stem cell function and development of myeloid
neoplasms.