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10.3892/ijmm.2015.2273 http://scihub22266oqcxt.onion/10.3892/ijmm.2015.2273 C4533777!4533777 !26151338     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26151338 &cmd=llinks): Failed to open stream: HTTP request failed! 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Pathogenesis of glucocorticoid-induced avascular necrosis: A microarray analysis of gene expression in vitro |pdf=|usr=}}{{26151338 }} gab.com Text Pathogenesis of glucocorticoid-induced avascular necrosis: A microarray analysis of gene expression in vitro JO: Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=26151338 #FOAvip Avascular necrosis of the femoral head (ANFH) occurs following exposure to corticosteroids, and the proliferative capacity of the mesenchymal stem cells (MSCs) belonging to ANFH was reduced. The previous studies indicate that microRNA (miRNA) has an important regulatory role during proliferation and osteogenic differentiation of MSCs. Therefore, MSCs were obtained from healthy adults, and were cultured and osteogenically?induced by different dexamethasone concentrations. The proliferation and osteogenic differentiation capacities were examined through observing cellular morphology, alkaline phosphatase and alizarin red; miRNA expression was investigated using an miRNA gene chip and miRNA of differential expressions were retrieved through a database to analyze its regulatory effect. Dexamethasone at a concentration of 10(?7) mol/l induced the proliferation and osteogenic differentiation of MSCs and resulted in evident miRNA expression profile changes. In total, 11 miRNAs were upregulated at 10(?7) mol/l while 6 were downregulated, and partial miRNA was identified to participate in the regulation of cell proliferation and cell apoptosis, MSC osteogenic differentiation, lipid metabolism and other processes. Twit Text FOAVip Pathogenesis of glucocorticoid-induced avascular necrosis: A microarray analysis of gene expression in vitro ????Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=26151338 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26151338 #FOAvip English Wikipedia <ref>{{Cite pmid|26151338 }}</ref> Pathogenesis of glucocorticoid-induced avascular necrosis: A microarray analysis of gene expression in vitro #MMPMID26151338 Bian Y ; Qian W ; Li H ; Zhao RC ; Shan WX ; Weng X Int J Mol Med 2015[Sep]; 36 (3 ): 678-84 PMID26151338 show ga Avascular necrosis of the femoral head (ANFH) occurs following exposure to corticosteroids, and the proliferative capacity of the mesenchymal stem cells (MSCs) belonging to ANFH was reduced. The previous studies indicate that microRNA (miRNA) has an important regulatory role during proliferation and osteogenic differentiation of MSCs. Therefore, MSCs were obtained from healthy adults, and were cultured and osteogenically?induced by different dexamethasone concentrations. The proliferation and osteogenic differentiation capacities were examined through observing cellular morphology, alkaline phosphatase and alizarin red; miRNA expression was investigated using an miRNA gene chip and miRNA of differential expressions were retrieved through a database to analyze its regulatory effect. Dexamethasone at a concentration of 10(?7) mol/l induced the proliferation and osteogenic differentiation of MSCs and resulted in evident miRNA expression profile changes. In total, 11 miRNAs were upregulated at 10(?7) mol/l while 6 were downregulated, and partial miRNA was identified to participate in the regulation of cell proliferation and cell apoptosis, MSC osteogenic differentiation, lipid metabolism and other processes. |Adult [MESH] |Cells, Cultured [MESH] |Dexamethasone/*adverse effects [MESH] |Femur Head Necrosis/*chemically induced/genetics/*pathology [MESH] |Gene Expression Regulation/*drug effects [MESH] |Glucocorticoids/*adverse effects [MESH] |Humans [MESH] |Mesenchymal Stem Cells/drug effects/metabolism/pathology [MESH] |MicroRNAs/*genetics [MESH] |Oligonucleotide Array Sequence Analysis [MESH]Pathogenesis of glucocorticoid-induced avascular necrosis: A microarra(epmc).pdf DeepDyve Pubget Overpricing