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2015 ; 6
(ä): 634
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Auxin is a long-range signal that acts independently of ethylene signaling on
leaf abscission in Populus
#MMPMID26322071
Jin X
; Zimmermann J
; Polle A
; Fischer U
Front Plant Sci
2015[]; 6
(ä): 634
PMID26322071
show ga
Timing of leaf abscission is an important trait for biomass production and
seasonal acclimation in deciduous trees. The signaling leading to organ
separation, from the external cue (decreasing photoperiod) to ethylene-regulated
hydrolysis of the middle lamellae in the abscission zone, is only poorly
understood. Data from annual species indicate that the formation of an auxin
gradient spanning the abscission zone regulates the timing of abscission. We
established an experimental system in Populus to induce leaf shedding
synchronously under controlled greenhouse conditions in order to test the
function of auxin in leaf abscission. Here, we show that exogenous auxin delayed
abscission of dark-induced leaves over short and long distances and that a new
auxin response maximum preceded the formation of an abscission zone. Several
auxin transporters were down-regulated during abscission and inhibition of polar
auxin transport delayed leaf shedding. Ethylene signaling was not involved in the
regulation of these auxin transporters and in the formation of an abscission
zone, but was required for the expression of hydrolytic enzymes associated with
cell separation. Since exogenous auxin delayed abscission in absence of ethylene
signaling auxin likely acts independently of ethylene signaling on cell
separation.