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10.3904/kjim.2000.15.3.218 http://scihub22266oqcxt.onion/10.3904/kjim.2000.15.3.218 C4531773!4531773!11242810     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Korean+J+Intern+Med 2000 ; 15 (3): 218-23 Nephropedia Template TP {{tp|p=11242810|t=2000. Gentamicin Induced Apoptosis of Renal Tubular Epithelial (LLC-PK1) Cells |pdf=|usr=}}{{11242810}} gab.com Text Gentamicin Induced Apoptosis of Renal Tubular Epithelial (LLC-PK1) Cells JO: Korean J Intern Med http://www.kidney.de/pget.php?&tw=1&pmid=11242810 #FOAvip Nephrotoxicity is a major limiting factor in the use of aminoglycoside antibiotics, the mechanisms for which are still speculative. To clarify the mechanisms of renal tubular cell death induced by aminoglycosides, we examined the renal proximal tubule-like cell line, LLC-PK1, after inducing apoptosis through a chronic treatment with gentamicin (GM). Changes in the expression of the Fas were also investigated. On flow cytometric analysis, 5.7 ± 3.3% of the control cells appeared in a region of decreased forward light scatter and increased side light scatter, where both indices represent the characteristics of apoptotic cell death. Compared to the control, treatment with 10 mM of GM for 15 days significantly increased the proportion of cells in the apoptotic region to 23.9 ± 8.5%. This finding was supported by electrophoretic analysis of the DNA extracted from the GM-treated cells, where a series of bands corresponding to integer multiples of 180 to 200 base pairs was visualized. However, the 15-day GM treatment did not cause a significant elevation in the expression of the 45 kD Fas protein, the cell surface molecule that stimulates apoptosis, by Western blot analysis.In conclusion, long-term exposure to GM induces apoptosis of the renal tubular epithelial cells, and this process may contribute to some of the aminoglycoside nephrotoxicities. Further studies are needed on the mechanism(s) of apoptosis induced by GM. Twit Text FOAVip Gentamicin Induced Apoptosis of Renal Tubular Epithelial (LLC-PK1) Cells ????Korean J Intern Med http://www.kidney.de/pget.php?&tw=1&pmid=11242810 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=11242810 #FOAvip English Wikipedia <ref>{{Cite pmid|11242810}}</ref> Gentamicin Induced Apoptosis of Renal Tubular Epithelial (LLC-PK1) Cells #MMPMID11242810 Choi KH; Kim TI; Chong DL; Lee HY; Han DS Korean J Intern Med 2000[Dec]; 15 (3): 218-23 PMID11242810show ga Nephrotoxicity is a major limiting factor in the use of aminoglycoside antibiotics, the mechanisms for which are still speculative. To clarify the mechanisms of renal tubular cell death induced by aminoglycosides, we examined the renal proximal tubule-like cell line, LLC-PK1, after inducing apoptosis through a chronic treatment with gentamicin (GM). Changes in the expression of the Fas were also investigated. On flow cytometric analysis, 5.7 ± 3.3% of the control cells appeared in a region of decreased forward light scatter and increased side light scatter, where both indices represent the characteristics of apoptotic cell death. Compared to the control, treatment with 10 mM of GM for 15 days significantly increased the proportion of cells in the apoptotic region to 23.9 ± 8.5%. This finding was supported by electrophoretic analysis of the DNA extracted from the GM-treated cells, where a series of bands corresponding to integer multiples of 180 to 200 base pairs was visualized. However, the 15-day GM treatment did not cause a significant elevation in the expression of the 45 kD Fas protein, the cell surface molecule that stimulates apoptosis, by Western blot analysis.In conclusion, long-term exposure to GM induces apoptosis of the renal tubular epithelial cells, and this process may contribute to some of the aminoglycoside nephrotoxicities. Further studies are needed on the mechanism(s) of apoptosis induced by GM. äGentamicin Induced Apoptosis of Renal Tubular Epithelial (LLC-PK1) Cel(epmc).pdf DeepDyve Pubget Overpricing