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2004 ; 19
(3
): 141-8
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Conformational transitions and glycation of serum albumin in patients with
minimal-change glomerulopathy
#MMPMID15481604
Hong SY
; Lee EY
; Yang JO
; Kim TY
; Kim EH
; Cheong MY
; Kim SH
; Cheong CJ
Korean J Intern Med
2004[Sep]; 19
(3
): 141-8
PMID15481604
show ga
BACKGROUND: There has been a lack of study on the structural changes of serum
albumin in patients with minimal change disease (MCD). To determine whether
glycation and/or conformational transitions of albumin are involved in the
pathogenesis of albuminuria, nine patients with MCD were enrolled in a
prospective follow-up study for comparison of these parameters in serum albumin
during the remission and relapse of nephrotic syndrome. METHODS: Circular
dichroism measurements were made with purified albumin. Ellipticities at each
wavelength were transformed to mean residue ellipticity. Monosaccharide
composition was analyzed by high-pH anion-exchange chromatography with pulsed
amperometric detection. RESULTS: There was no difference in the proportions of
alpha-helix, beta-conformation, and beta-turn of albumin between the sera of
control patients and those with nephrotic syndrome. However, the proportion of
the random configuration was slightly higher in the plasma albumin of patients in
relapse than in those in remission. The proportion of the random configuration
was lower in the albumin of the serum than in the urine of patients with
nephrotic syndrome, but there was no difference in the proportions of
alpha-helix, beta-conformation, and beta-tum of albumin between their plasma and
urine. CONCLUSION: Our results suggest that conformational changes in albumin are
involved in albuminuria in patients with MCD.