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10.1002/0471143030.cb0429s67

http://scihub22266oqcxt.onion/10.1002/0471143030.cb0429s67
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C4529119!4529119!26061244
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suck abstract from ncbi


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pmid26061244      Curr+Protoc+Cell+Biol 2015 ; 67 (ä): 4.29.1-4.29.13
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  • Polarized Fluorescence Microscopy to Study Cytoskeleton Assembly and Organization in live cells #MMPMID26061244
  • McQuilken M; Mehta SB; Verma A; Harris G; Oldenbourg R; Gladfelter AS
  • Curr Protoc Cell Biol 2015[]; 67 (ä): 4.29.1-4.29.13 PMID26061244show ga
  • The measurement of not only the location but also the organization of molecules in live cells is crucial to understanding diverse biological processes. Polarized light microscopy provides a nondestructive means to evaluate order within subcellular domains. When combined with fluorescence microscopy and GFP-tagged proteins, the approach can reveal organization within specific populations of molecules. This unit describes a protocol for measuring the architectural dynamics of cytoskeletal components using polarized fluorescence microscopy and OpenPolScope open-access software (www.openpolscope.org). The protocol describes installation of linear polarizers or a liquid crystal (LC) universal compensator, calibration of the system, polarized fluorescence imaging, and analysis. The use of OpenPolScope software and hardware allows for reliable, user-friendly image acquisition to measure and analyze polarized fluorescence.
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